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Molecular Biology
High-Fidelity DNA Polymerase
New England Biolabs’ Q5™ High-Fidelity DNA Polymerase offers robust amplification across the broadest range of amplicons, including those that are GC- and AT-rich.
The polymerase is fused to the processivity-enhancing Sso7d DNA binding domain, which improves fidelity (more than 50-fold higher than Taq), speed, and performance reliability (amplifying targets up to 20 kb long with extension times as low as 10 seconds per kb). The Q5 buffer system has also been optimized to provide robust performance across both high-GC and high-AT regions, as well as other challenging amplicons.
 
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Established in the mid-1970s as a cooperative laboratory of experienced scientists, New England Biolabs is a world leader in the production and supply of reagents for the life science industry. NEB now offers the largest selection of recombinant and native enzymes for genomic research and continues to expand its product offerings into areas related to proteomics and drug discovery.