The InBreath bioreactors for tubular organ regeneration is designed for tissue engineering and regenerative medicine research applications using trachea, esophagus, intestines, blood vessels, or any hollow organ.
The bioreactor is engineered for cell seeding and culturing on both
intraluminal and extraluminal surfaces of a tubular matrix. A polymer-based
culture chamber houses the scaffold and culture medium for the entire
duration of the organ generation procedure. An organ scaffold is mounted to
the corresponding scaffold holder of appropriate diameter. Each holder
features a reduced diameter central portion which functions to expose the
intraluminal surface of the matrix for cell seeding and culturing. Secondary
elements or “paddles” moving with the scaffold holder produce continuous
mixing of the culture medium to increase oxygenation and mass transport. The
cell/scaffold construct is rotated on the holder by a brushless DC motor
(0-5 rpm adjustable) which is completely separated from the culture
compartment. A co-axial conduit links the inner chamber to the external
environment through an interface at the chamber wall. The detachable
connection between the motor unit and the culture chamber allows the latter
to be removed for maintenance while the motor unit remains stationed in the
incubator. An external control unit regulates and monitors rotation.
Autoclavability, ease of handling under sterile conditions, reliability and
precision ensures full compatibility of the device with GLP rules.