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Molecular Biology
Methyl-Seq Library Prep
The NEXTflex Methyl-Seq 1 kit is designed to enrich and prepare single, paired-end and multiplexed methylated DNA libraries for sequencing using Illumina MiSeq, GAIIx, and HiSeq platforms while allowing for the multiplexing of up to 96 samples facilitating a methylome-level assessment of genomic DNA.
NEXTflex Methyl-Seq Kit utilizes versatile MeDIP or MeCAP protocols for detection of methylated DNA allowing the user to easily assess the methylation state of the genome, quantify absolute DNA methylation levels, and identify differentially methylated regions. The NEXTflex Methyl-Seq 1 Kit includes "Enhanced Adapter Ligation Technology" resulting in library preps with a larger number of unique sequencing reads. This specially designed NEXTflex ligation enzymatic mix allows users to perform ligations with longer adapters and better ligation efficiencies. This kit also uses a completely gel-free protocol making the workflow compatible with liquid handler automation. In addition, NEXTflex DNA Barcodes can be purchased separately in sets of 12, 24, 48 or 96 unique barcodes for multiplexing.
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