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Molecular Biology
Microbial Genome Finishing Module
The CLC Microbial Genome Finishing Module is an add-on module to CLC Genomics Workbench, which has been developed to help finish the assembly of small genomes.
CLC Microbial Genome Finishing Module is a collection of tools with different functionalities to identify, visualize, and solve problems in genome assemblies. The tools are: - Align Contigs. Aligns contigs to a reference sequence or, in the absence of a reference, to the contigs themselves. - Analyze Contigs. Analyzes the contig read mappings for possible misassemblies, single strandedness, coverage, broken pairs, and unaligned ends. - Collect Paired Reads Statistics. Detects paired reads that map to separate contigs. - Create Amplicons. Places amplicon annotations on sequences. Used before the Primer Creator to subdivide regions of interest into fragments of suitable sizes. - Create Primers. Automates primer design for re-sequencing purposes. - Add Reads to Contigs. Adds additional sequence data to existing contigs. - Sample Reads. Allows a user-defined reduction in the number of reads. - Find Sequence. Searches for names, sequence strings, or annotations in de novo assemblies, mappings, and sequencing data. - Reassemble Regions. Reassembles selected regions in contigs. Useful for solving small misassemblies. - Extend Contigs. Extends contigs with existing reads.
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