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Cell Culture
Nunclon Vita surface
The Thermo Fisher Scientific Nunclon Vita stem cell culture surface is deisnged to enable direct attachment, colony formation and growth of human embryonic stem (ES) cells and induced pluripotent stem cells (iPS), without the need for feeder cells or matrix coatings.
In media supplemented with a Rho-associated kinase (ROCK) inhibitor, human ES cells can be cultured on the Nunclon Vita Surface for at least 10 passages without any loss of pluripotency, or karyotype changes. As a result, Nunclon Vita offers greater control over culturing of stem cells and providing a more stable supply of cells for downstream applications. By eliminating matrix coatings and feeder cells, the Nunclon Vita surface reduces culture variability and is less labor-intensive than cell lines that require matrix coatings or feeder cells. The Nunclon Vita surface treatment is much more adaptable for scalable cell expansion as cells grow directly on the polystyrene surface. In addition, the new surface works in synergy with a ROCK inhibitor for expansion of human ES cells, IPS, as well as human embryonic kidney cells (HEK) to enable enzymatic and mechanical passage protocols.
 
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Thermo Fisher Scientific Inc. is the world leader in serving science, enabling customers to make the world healthier, cleaner and safer. With 2008 revenues of $10.5 billion, the company has approximately 34,000 employees and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings.

Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, the company helps solve analytical challenges from routine testing to complex research and discovery.

The Thermo Scientific brand represents a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables, and reagents to enable integrated laboratory workflow solutions.

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Together, the company offers the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike.

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Thermo Fisher Scientific, Inc.
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Waltham, MA 02454 USA
Tel: +1 781-622-1000
Fax: +1 781-622-1207