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Electrophoresis, Proteomics, Separations
G Biosciences’ Perfect-FOCUS is designed to clean and concentrate protein samples that give poor protein spot resolution during two-dimensional (2-D) electrophoresis. Protein samples containing interfering agents, including ionic detergents, metal ions, substrates, substrate analogs, inhibitors, and other charged molecules, routinely result in smeared or low-resolution gels. Protein solutions are treated with Perfect-FOCUS that quantitatively precipitates the proteins, which are subsequently collected by centrifugation, according to G Biosciences. The non-protein interfering agents are washed away and the protein pellet is ready to be solubilized in an appropriate sample loading buffer. The collected protein has conductivity of <50 s and is substantially free from non-protein agents, which improves spot resolution and greatly reduces spot streaking, the company reports. Perfect-FOCUS–treated protein samples are suitable for isoelectric focusing (IEF) and 2-D gel electrophoresis applications.
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