Chromatography,
Proteomics,
UV-Vis
Photodiode array detector for the TDAmax size exclusion chromatography system
A new photodiode array (PDA) detector for the TDAmax size exclusion
chromatography system from Viscotek provides a flexible tool for the rapid
analysis of a wide range of proteins and biopolymers. These include
identifying protein conjugates and complexes, aggregation and stability and
determining concentration ratios. The TDAmax is a comprehensive GPC/SEC
system with an integrated triple or tetra detector array. By capturing
absorption spectra across the UV-Vis wavelength range, the new PDA detector
provides a fingerprint, the full absorption chromatogram, of each ‘time
slice’ of the sample as it elutes from the column. Measurement times are
30–40 minutes, making the system highly productive, the company reports. UV
detection is used routinely to identify protein and polymer species as they
elute, and can differentiate between components when other detection methods
cannot. The new PDA detector captures data across a range of 190–500 nm,
allowing users to select wavelengths of interest after the analysis has been
carried out. Viscotek’s OmniSEC software package allows data to be displayed
as information-rich three-dimensional (3-D) images that simplify
interpretation, according to the company.
