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Immunoaffinity: Protein Detection, Western Blot
Pierce Fast Semi-Dry Blotter and Semi-Dry Transfer Buffer
Thermo Scientific has announced a pair of products designed to accelerate Western blotting procedures. The Pierce Fast Semi-Dry Blotter enables the rapid transfer of proteins or nucleic acids from a gel to PVDF or nitrocellulose membranes. When used with the new Pierce Fast Semi-Dry Transfer Buffer, efficient protein transfer occurs within 7 to 10 minutes, according to the company The Pierce Fast Semi-Dry Blotter can transfer up to four mini-gels at once. The system can also be used for conventional semi-dry transfer using other buffers and protocols. The Pierce Fast Semi-Dry Transfer Buffer is supplied as a 10X concentrate that is diluted with deionized water before use. It does not require methanol. The buffer can also be used with other commercially available semi-dry transfer units.
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