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Cell Culture, Nucleic-acid technologies: PCR
PluriPCR kit
AMSBIO's PluriPCR Kit is designed to be a quantitative, easy-to-use, and reliable assay of five genes strongly specific to pluripotency.
These genes: Oct-3/4, Nanog, DNMT3b, Dppa4, and Rex1 are expressed by human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC); all are sharply down-regulated during differentiation. The kit will allow stem cell scientists to accurately and unambiguously define the pluripotency of a cell line using existing real-time quantitative PCR equipment. PluriPCR uses five key genes, in combination with a unique normalization method, which gives a reliable and quantitative readout for the pluripotency of a cell line. The kit is suited for all aspects of pluripotent stem cell research: measure if culture conditions are resulting in the loss of pluripotency, to assess successful nuclear reprogramming of iPS cells, and even as a release assay in the manufacture and clinical development of pluripotent stem cells. The PluriPCR kit is optimized for routine quality control using either two-step or one-step real-time quantitative PCR methods. The assay kit combines multiple pluripotency and housekeeping markers and an analysis tool to assess the pluripotency of your cell line. The resultant PluriPCR Index is a simple method to understand if your pluripotent stem cells are differentiating.
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Founded in 1987, AMS Biotechnology (AMSBIO) is recognised as a leading international provider of unique & innovative products & custom services for life sciences research. The AMSBIO range includes over 23,000 polyclonal & monoclonal antibodies, peptides, recombinant proteins, extracellular matrix, molecular detection reagents, & tissue DNA, RNA, protein & microarray products. Key research areas include: apoptosis, cell invasion & migration, cell signaling, DNA damage, electrophoresis, glycobiology, posttranslational modification & stem cell biology.