Fluidigm's C1 Single-Cell AutoPrep System is designed to enable a researcher to isolate and process individual cells rapidly and reliably for genomic analysis.
Researchers can isolate cells, extract RNA, and then reverse transcribe and
preamplify mRNA transcripts automatically to enable detection and analysis
of cell activity.
The system's workflow is initiated by loading a sample of cells in solution
into the C1 microfluidic chip in a single pipetting step, then directing the
C1 system to rapidly and automatically isolate up to 96 individual cells
into individual chambers for preparation. After loading, researchers can
choose an “in-process” quality control checkpoint to verify the number of
captured cells and distinguish live from dead cells to preserve data
integrity. The workflow then proceeds with a rapid “on-chip” cell lysis
without RNA purification, reverse transcription, and preamplification
without hands-on reagent mixing and sample transfer. The final preamplified
cDNA product is thereafter harvested to collection wells for transfer to the
BioMark HD System for quantitative PCR analysis.
Fluidigm develops, manufactures, and markets proprietary Integrated Fluidic
Circuit (IFC) systems that significantly improve productivity in life
science research. Fluidigm’s IFCs enable the simultaneous performance of
thousands of sophisticated biochemical measurements in extremely minute
volumes. These “integrated circuits for biology” are made possible by
miniaturizing and integrating liquid handling components on a single
microfluidic device (IFC). Fluidigm’s IFC systems, consisting of
instrumentation, software and single-use IFCs, increase throughput, decrease
costs and enhance sensitivity compared to conventional laboratory systems.
Fluidigm products have not been cleared or approved by the Food and Drug
Administration for use as a diagnostic and are only available for research
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