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Molecular Biology
Single-Cell AutoPrep System
Fluidigm's C1 Single-Cell AutoPrep System is designed to enable a researcher to isolate and process individual cells rapidly and reliably for genomic analysis.
Researchers can isolate cells, extract RNA, and then reverse transcribe and preamplify mRNA transcripts automatically to enable detection and analysis of cell activity. The system's workflow is initiated by loading a sample of cells in solution into the C1 microfluidic chip in a single pipetting step, then directing the C1 system to rapidly and automatically isolate up to 96 individual cells into individual chambers for preparation. After loading, researchers can choose an “in-process” quality control checkpoint to verify the number of captured cells and distinguish live from dead cells to preserve data integrity. The workflow then proceeds with a rapid “on-chip” cell lysis without RNA purification, reverse transcription, and preamplification without hands-on reagent mixing and sample transfer. The final preamplified cDNA product is thereafter harvested to collection wells for transfer to the BioMark HD System for quantitative PCR analysis.
 
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Fluidigm develops, manufactures, and markets proprietary Integrated Fluidic Circuit (IFC) systems that significantly improve productivity in life science research. Fluidigm’s IFCs enable the simultaneous performance of thousands of sophisticated biochemical measurements in extremely minute volumes. These “integrated circuits for biology” are made possible by miniaturizing and integrating liquid handling components on a single microfluidic device (IFC). Fluidigm’s IFC systems, consisting of instrumentation, software and single-use IFCs, increase throughput, decrease costs and enhance sensitivity compared to conventional laboratory systems. Fluidigm products have not been cleared or approved by the Food and Drug Administration for use as a diagnostic and are only available for research use.

Fluidigm Corporation
7000 Shoreline Court, Suite 100
South San Francisco, CA 94080
Phone: 1-866-358-4354
Fax: 650-871-7152
http://www.fluidigm.com