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Nucleic-acid technologies: PCR
SsoFast EvaGreen control assay
The SsoFast EvaGreen control assay from Bio-Rad Laboratories is a prepackaged kit that helps labs verify their real-time PCR instrument performance.
Large labs experiencing frequent personnel changes can use the control assay to train new users on real-time PCR experiment set up, instrument operation, and data analysis, and to verify that all users can obtain consistent results. The assay provides: • Broad dynamic range over six orders of magnitude, from 102 to 108 copies of template • High efficiency and R2 (greater than 90% efficiency and R2 > 0.99 with good laboratory technique) • Fast run times (50 min including a melt curve on the CFX96 real-time detection system) The kit contains enough reagents to run a standard curve three times and includes control template DNA and primers, SsoFast EvaGreen supermix, nuclease-free water, and recommended cycling protocols as well as instructions on how to set up a standard curve.
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Bio-Rad Laboratories is a corporation providing a broad range of innovative tools and services to the life science research and clinical diagnostics markets. Founded in 1952, Bio-Rad has a global team of more than 6,500 employees and serves more than 85,000 research and industry customers worldwide through its global network of operations. Throughout its existence, Bio-Rad has built strong customer relationships that advance scientific research and development efforts and support the introduction of new technology used in the growing fields of genomics, proteomics, drug discovery, food safety, medical diagnostics, and more.