The Stratagene Absolutely RNA Microprep Kit, from Agilent Automation Solutions (originally from Velocity11), allows high-throughput isolation of total RNA from small samples of cultured cells. This method eliminates toxic phenol-chloroform extractions and time-consuming ethanol precipitations by selectively binding RNA from cell lysate on 96-well silica fiber matrix plates. The RNA isolation protocol requires extensive manual processing including repetitive pipetting, washing, vacuum filtration and centrifugation steps. Using Agilent's VWorks Automation Control Software, a script was written for the Bravo Platform to aspirate, dispense, mix and transfer sample from a source 96-well tissue culture plate to the binding and collection plates of the Absolutely RNA 96 kit; as well as to automate sample wash steps using a vacuum filtration unit accessory on the Bravo Platform. The Bravo Platform is shown to provide a reliable, efficient solution for automatically handling the multiple steps in total RNA isolation using the Absolutely RNA 96 Microprep kit, Agilent reports. Additionally, the unique, open design of the Bravo Platform permits access from all sides for simple integration with other devices and unencumbered standalone use.
Agilent Technologies is the world’s premier measurement company and a technology leader in communications, electronics, life sciences and chemical analysis. Agilent has two primary business segments: Bio-Analytical Measurement and Electronic Measurement. The company has 19,600 employees and serves customers in more than 110 countries. Agilent had revenue of $5.8B in fiscal year 2008.