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Cell Biology
Synthetic mRNA Products for Reprogramming Human Cells
AMSBIO's highly modified synthetic 5-prime capped mRNA products are designed for reprogramming human cells.
These new ready-to-use products provide a simple, safe non-integrating
methodology for reprogramming cell fate based on the administration of
synthetic modified mRNA that greatly increases efficiency whilst reducing
the innate antiviral response of viral protocols.
Clinical applications of induced pluripotent stem cells (iPSCs) have
traditionally been limited by the low efficiency of iPSC derivation and the
fact that most protocols modify the genome to effect cellular reprogramming.
Moreover, safe and effective means of directing the fate of patient-specific
iPSCs toward clinically applicable cell types are not possible by viral
methods.
Today the company can provide ready-to-use highly modified mRNAs including
Kfl4, c-Myc, Oct4, Sox2, LIN28 mRNA, and eGFP control, in customised formats
for your research. These proven synthetic adaptations of 5-prime, 3-prime
and internal mRNA structures enable reprogramming of multiple human cell
types to pluripotency with conversion efficiencies that greatly surpass
established methods. Furthermore this same technology can be used to
efficiently direct the differentiation of mRNA-induced pluripotent stem
cells into terminally differentiated cells and can be employed as an
alternative to DNA-based gene therapy [2].
This technology represents a safe, non-mutagenic and efficient strategy for
somatic cell reprogramming and directing cell fate that has broad
applicability for basic research, disease modeling, tissue-engineering and
regenerative medicine.
This Product is made by:
Founded in 1987, AMS Biotechnology (AMSBIO) is recognised as a leading international provider of unique & innovative products & custom services for life sciences research. The AMSBIO range includes over 23,000 polyclonal & monoclonal antibodies, peptides, recombinant proteins, extracellular matrix, molecular detection reagents, & tissue DNA, RNA, protein & microarray products. Key research areas include: apoptosis, cell invasion & migration, cell signaling, DNA damage, electrophoresis, glycobiology, posttranslational modification & stem cell biology.
