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Fluorescence Microscopy, Imaging
TIRF microscopy objectives
Olympus' high-performance total internal reflection fluorescence (TIRF) microscope objectives are designed to support scientists in their quest for knowledge about living cells. The 150x ultra-magnification objective offers a numerical aperture (NA) of 1.45 for optimal magnification, resolution, light gathering ability and imaging performance. New 60x and 100x oil immersion objectives have NAs of 1.49. Olympus now offers five specifically designed TIRF objectives. Olympus also offers a 150x magnification TIRF lens, along with the 100x NA 1.65. The 150x UAPON NA 1.45 objective is optimized for single molecule TIRF applications. It delivers the high magnification and low background noise necessary for visualizing challenging specimens. The objective delivers crisp images from 340-nm ultraviolet (UV) to near-infrared wavelengths and comes with a correction collar to help compensate for aberrations caused by coverglass thickness or variations in immersion media temperatures. The 100x UAPON NA 1.49 objective also is newly enhanced with a correction collar, and has been updated to include near-UV performance. The 60x APON NA 1.49 provides bright high-resolution TIRF images because of its improved point spread function. All Olympus TIRF objectives can take full advantage of the recently introduced Olympus cell^TIRFTM illuminator, which offers four-color, simultaneous, fully motorized control of TIRF excitation along with extraordinary ease of use.
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Olympus is a precision technology leader, designing and delivering innovative solutions in its core business areas: Medical and Surgical Products, Life Science Imaging Systems, Industrial Testing Instruments and Cameras and Audio Products.

Olympus works collaboratively with its customers and affiliates worldwide to leverage R&D investment in precision technology and manufacturing processes across diverse business lines. These include:

• Gastrointestinal endoscopes, accessories, and minimally invasive surgical products;

• Advanced research, clinical and educational microscopes and clinical digital imaging systems;

• Industrial research, engineering, test, inspection and measuring instruments; and

• Digital cameras and voice recorders.

Olympus serves the healthcare field with integrated product solutions and financial, educational and consulting services that help customers to efficiently, reliably and more easily achieve exceptional results. Olympus develops breakthrough technologies with revolutionary product design and functionality for the consumer and professional photography markets, and also is the leader in gastrointestinal endoscopy and clinical and educational microscopes. For more information, visit