Vector Laboratories' Neurobiotin 488 Tracer is a new tri-functional molecule designed for neuronal tracing and cell filling.
It is an amine containing derivative of biotin conjugated with a bright green
fluorophore (similar in fluorescence to fluorescein, Cy2 or AlexaFluor 488).
The molecule is transported along axons and fine projections and readily
fixed with standard aldehyde based fixatives. It can be viewed directly via
fluorescence microscopy or subsequently detected with a choice of
(strept)avidin reagents for light microscopy and archiving of the
preparation. Neurobiotin 488 is compatible with patch clamp applications and
pressure injection methods. Neurobiotin 488 is provided as Cat. No.
SP-1125 in a 2 mg package and is also accompanied with "Guidelines for
Intracellular Labeling" and a Certificate of Analysis.