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Imaging
cell^frap system for simultaneous imaging and photo-manipulation
The cell^frap module for the Olympus xcellence live cell imaging and manipulation system enables users to perform fluorescence recovery after photo-bleaching (FRAP), inverse FRAP (iFRAP), fluorescence loss in photobleaching (FLIP), and fluorescence loss after photobleaching (FLAP) experiments.
The cell^frap system is also designed for advanced processes such as photo-conversion, photo-activation, pattern bleaching, laser cutting and trapping. The module includes its own dedicated laser system and light-path, allowing simultaneous imaging and photo-manipulation. These techniques are controlled by the xcellence software interface. The cell^frap module is introduced into the microscope via a dedicated light-path and can incorporate two separate laser lines or a laser combiner. This enables simultaneous imaging and photo-manipulation, providing full integration of FRAP with epi-fluorescence, TIRFM, and spinning disk confocal microscopy. The system scans just the region of interest, achieving a quick rate of bleaching. This makes it possible to design complex protocols that investigate multiple regions of interest. In addition, intelligent hardware-based synchronization allows µ second switching between bleaching and imaging, meaning that initial frames can be acquired as early as 150 µs after the FRAP experiment ends, providing accurate quantitative data as well as high quality images.
 
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Olympus is a precision technology leader, designing and delivering innovative solutions in its core business areas: Medical and Surgical Products, Life Science Imaging Systems, Industrial Testing Instruments and Cameras and Audio Products.

Olympus works collaboratively with its customers and affiliates worldwide to leverage R&D investment in precision technology and manufacturing processes across diverse business lines. These include:

• Gastrointestinal endoscopes, accessories, and minimally invasive surgical products;

• Advanced research, clinical and educational microscopes and clinical digital imaging systems;

• Industrial research, engineering, test, inspection and measuring instruments; and

• Digital cameras and voice recorders.

Olympus serves the healthcare field with integrated product solutions and financial, educational and consulting services that help customers to efficiently, reliably and more easily achieve exceptional results. Olympus develops breakthrough technologies with revolutionary product design and functionality for the consumer and professional photography markets, and also is the leader in gastrointestinal endoscopy and clinical and educational microscopes. For more information, visit www.olympusamerica.com.