The cell^frap module for the Olympus xcellence live cell imaging and manipulation system enables users to perform fluorescence recovery after photo-bleaching (FRAP), inverse FRAP (iFRAP), fluorescence loss in photobleaching (FLIP), and fluorescence loss after photobleaching (FLAP) experiments.
The cell^frap system is also designed for advanced processes such as
photo-conversion, photo-activation, pattern bleaching, laser cutting and
trapping. The module includes its own dedicated laser system and light-path,
allowing simultaneous imaging and photo-manipulation. These techniques are
controlled by the xcellence software interface.
The cell^frap module is introduced into the microscope via a dedicated
light-path and can incorporate two separate laser lines or a laser combiner.
This enables simultaneous imaging and photo-manipulation, providing full
integration of FRAP with epi-fluorescence, TIRFM, and spinning disk confocal
The system scans just the region of interest, achieving a quick rate of
bleaching. This makes it possible to design complex protocols that
investigate multiple regions of interest. In addition, intelligent
hardware-based synchronization allows µ second switching between bleaching
and imaging, meaning that initial frames can be acquired as early as 150 µs
after the FRAP experiment ends, providing accurate quantitative data as well
as high quality images.
Olympus is a precision technology leader, designing and delivering innovative
solutions in its core business areas: Medical and Surgical Products, Life
Science Imaging Systems, Industrial Testing Instruments and Cameras and
Olympus works collaboratively with its customers and affiliates worldwide to
leverage R&D investment in precision technology and manufacturing processes
across diverse business lines. These include:
• Gastrointestinal endoscopes, accessories, and minimally invasive surgical
• Advanced research, clinical and educational microscopes and clinical digital
• Industrial research, engineering, test, inspection and measuring
• Digital cameras and voice recorders.
Olympus serves the healthcare field with integrated product solutions and
financial, educational and consulting services that help customers to
efficiently, reliably and more easily achieve exceptional results. Olympus
develops breakthrough technologies with revolutionary product design and
functionality for the consumer and professional photography markets, and
also is the leader in gastrointestinal endoscopy and clinical and
educational microscopes. For more information, visit www.olympusamerica.com.