Bio-Rad introduces the V3 Western Workflow™, providing increased speed and confidence and improving quantitation compared to your traditional western blotting protocol. Learn how to improve your blotting results here.
Video Date: April 01 2012 Run Time:
Coupling Flow Cytometry With an Expanded Palette of Fluorescent Proteins to Enhance Gene Expression Analysis
Free Web Event
Tuesday, April 17, 2012
10 AM PDT / 1 PM EDT / 6 PM BST
Advances in flow cytometry and a full spectrum of fluorescent proteins now available allow biomedical researchers to more quickly, easily and affordably leverage this technology in gene expression analysis. Fluorescent proteins have come a long way since the original application of Green Fluorescent Protein (GFP) for the detection of gene expression. Fluorescent proteins now span the entire spectrum from short violet to long red, and can be used to study a wide variety of cellular phenomena. Personal flow cytometry simplifies the detection of fluorescently tagged genes incorporated into cells.
In the first segment of this webinar, we’ll review how fluorescent proteins and flow cytometry are utilized in gene expression analysis. The second portion will discuss strategies for the analysis of gene expression in angiosperms including a unique method for preparation of plant nuclei suspensions, the combination of natural or transgenic expression of fluorescence to identify specific cell types, and selective purification using cell sorting for subsequent gene expression analysis. The third portion will discuss how bacterial strains expressing fluorescent protein fusion constructs can be used to characterize the environment – gene regulation relationship and to quantify populations according to the resulting gene expression.
In this webinar you will learn:
- How flow cytometry techniques and a full spectrum of fluorescent proteins enable more efficient gene expression analysis.
- How the application of a gene expression workflow involving upstream flow cytometry for targeting, subsequent sorting for purification and downstream molecular analysis can speed gene expression analysis for different types of cells, including plant, bacterial and mammalian.
- How the BD Accuri C6 flow cytometer can be easily integrated into the laboratory for fast analysis, even for those with little flow cytometry experience
Moderator:
Patrick C.H. Lo, Ph.D.
Associate Editor, BioTechniques
Speakers:
Bill Telford
Director of the Flow Cytometry Core Laboratory in the NCI Experimental Transplantation and Immunology Branch
David Galbraith
Professor of Plant Sciences within the BIO5 Institute at the University of Arizona
Tim Cooper
Assistant Professor at the University of Houston
Grant Howes
Director of Marketing, Personal Flow Cytometry Platforms / BD - Accuri Cytometers
Sponsored by:
Register Now:
Signal Amplification for Immunocytochemistry And In Situ Hybridization: New Applications in High Content Screening
Free Web Event
Available on Demand
Tyramide Signal Amplification™ (TSA) is an enzymatic technique widely used in fixed cell assays such as immunocytochemistry and in situ hybridization to enhance sensitivity and specificity. In most cases, the technique improves sensitivity by 2-3 orders of magnitude, and some researchers report detection of single copy molecules. When applied to high content screening and analysis (HCS / HCA), TSA extends the quantitative range of the assay and improves reliability of detection for low abundance analytes or weakly associated primary antibodies. Please join us to learn about PerkinElmer’s signal amplification technology, and discover how TSA can improve your cellular imaging results.
In this webinar, you will:
- Review signal amplification methods for histochemistry and cytochemistry
- Learn about the application of signal amplification to HCA/HCS and PerkinElmer's new HCA ImagAmp™ reagent kits
- Get tips for adding signal amplification to your assay
- Be able to ask your questions to a panel of experts
Moderator:
Patrick C.H. Lo, Ph.D.
Associate Editor,
BioTechniques
Speakers:
John J. Shacka, Ph.D.
Assistant Professor in the Department of Pathology,
Neuropathology Division,
University of Alabama at Birmingham
Aaron Risinger
Imaging Applications Specialist,
PerkinElmer
Sponsored by:
Beyond the Diffraction Limit: Advances and Applications of Super-Resolution Imaging
Free Web Event
Wednesday, March 28, 2012
1 PM EDT / 10 AM PDT / 5 PM GMT
Super-resolution imaging is expanding our understanding of cellular structures and protein dynamics. First suggested more than 20 years ago, fluorescence techniques capable of resolution in the tens of nanometer range are becoming increasingly commonplace today. In this special web seminar, viewers will learn the basic principles behind several of the most commonly used super-resolution imaging techniques (STED, PALM, STORM, and GSD), hear specific examples where super-resolution approaches have provided unique biological insights, and get a glimpse of what the future holds for super-resolution studies.
Moderator:
Nathan Blow, Ph.D
Editor-in-Chief, BioTechniques
Speakers:
Joerg Bewersdorf
Assistant Professor of Cell Biology, Yale University
Dr. Bewersdorf and his team are developing new fluorescence microscopy techniques with spatial and/or temporal resolutions going far beyond current technology, and applying these to a diverse set of biological questions.
Catherine Galbraith
Senior Research Fellow, National Institutes of Health, Section on Organelle Biology. “Localizing Molecules to Visualize Biology”
Dr. Galbraith’s studies leverage super-resolution imaging approaches (including PALM and iPALM) to study fundamental mechanisms of cell fate, brain wiring, and synaptogenesis.
Markus Sauer
Professor, Julius-Maximilians-Universitat Wurzburg
Dr. Sauer’s talk will detail recent work from his lab on super-resolution imaging by dSTORM using standard probes with a specific focus on probe requirements for fixed and live-cell super-resolution imaging.
Sponsored by:
Register Now:
Principles and Applications of Multiphoton Imaging
Free Web Event
Wednesday, July 27, 2011
12 PM ET / 9 AM PT / 4 PM GMT / 5 PM BST
The advent of multiphoton microscopy has enabled researchers to image deeper, with greater resolution and less background, leading to new scientific insights into topics ranging from stem cell biology to the cellular effects of human disease. In this webinar, you will learn about the latest approaches and applications of multiphoton imaging. From presentations on the basic principles of multiphoton microscopy to descriptions of specialized applications such as FRET and intravital imaging, this webinar will answer your multiphoton microscopy questions and provide ideas to better utilize this powerful imaging technology for your research efforts.
In this webinar, you will:
- Learn the basic principles behind multiphoton imaging
- Discover the strengths and weakness of multiphoton microscopy
- Learn about the development of dual laser multiphoton microscopy and its use in FRET experiments
- Discover how multiphoton microscopy is used for intravital imaging
- Be able to ask your questions to a panel of experts
Moderator:
Patrick C.H. Lo, Ph.D.
Associate Editor, BioTechniques
Speakers:
Simon C. Watkins, Ph.D
Professor and Vice Chairman Cell Biology and Physiology, Center for Biologic Imaging, University of Pittsburgh
Tomasz Zal, Ph.D.
Assistant Professor, Department of Immunology, University of Texas, MD Anderson Cancer Center
Janos Peti-Peterdi, M.D., Ph.D.
Professor, Department of Physiology and Biophysics, Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California
Sponsored by:
Register FREE today at:
