Free Web Event
Available on Demand
Tyramide Signal Amplification™ (TSA) is an enzymatic technique widely used in fixed cell assays such as immunocytochemistry and in situ hybridization to enhance sensitivity and specificity. In most cases, the technique improves sensitivity by 2-3 orders of magnitude, and some researchers report detection of single copy molecules. When applied to high content screening and analysis (HCS / HCA), TSA extends the quantitative range of the assay and improves reliability of detection for low abundance analytes or weakly associated primary antibodies. Please join us to learn about PerkinElmer’s signal amplification technology, and discover how TSA can improve your cellular imaging results.
In this webinar, you will:
- Review signal amplification methods for histochemistry and cytochemistry
- Learn about the application of signal amplification to HCA/HCS and PerkinElmer's new HCA ImagAmp™ reagent kits
- Get tips for adding signal amplification to your assay
- Be able to ask your questions to a panel of experts
Moderator:
Patrick C.H. Lo, Ph.D.
Associate Editor,
BioTechniques
Speakers:
John J. Shacka, Ph.D.
Assistant Professor in the Department of Pathology,
Neuropathology Division,
University of Alabama at Birmingham
Aaron Risinger
Imaging Applications Specialist,
PerkinElmer
Sponsored by:
Principles and Applications of Multiphoton Imaging
Free Web Event
Wednesday, July 27, 2011
12 PM ET / 9 AM PT / 4 PM GMT / 5 PM BST
The advent of multiphoton microscopy has enabled researchers to image deeper, with greater resolution and less background, leading to new scientific insights into topics ranging from stem cell biology to the cellular effects of human disease. In this webinar, you will learn about the latest approaches and applications of multiphoton imaging. From presentations on the basic principles of multiphoton microscopy to descriptions of specialized applications such as FRET and intravital imaging, this webinar will answer your multiphoton microscopy questions and provide ideas to better utilize this powerful imaging technology for your research efforts.
In this webinar, you will:
- Learn the basic principles behind multiphoton imaging
- Discover the strengths and weakness of multiphoton microscopy
- Learn about the development of dual laser multiphoton microscopy and its use in FRET experiments
- Discover how multiphoton microscopy is used for intravital imaging
- Be able to ask your questions to a panel of experts
Moderator:
Patrick C.H. Lo, Ph.D.
Associate Editor, BioTechniques
Speakers:
Simon C. Watkins, Ph.D
Professor and Vice Chairman Cell Biology and Physiology, Center for Biologic Imaging, University of Pittsburgh
Tomasz Zal, Ph.D.
Assistant Professor, Department of Immunology, University of Texas, MD Anderson Cancer Center
Janos Peti-Peterdi, M.D., Ph.D.
Professor, Department of Physiology and Biophysics, Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California
Sponsored by:
Register FREE today at:
Free Web Event
Available on Demand
Finding answers to the most pressing questions in areas such as neuroscience, cancer research and developmental biology often requires detection of targeted protein, mRNA or microRNA in cells and tissues at very low levels, in some cases down to a single copy. Tyramide Signal Amplification (TSA) dramatically improves the sensitivity of these assays while preserving or even enhancing resolution. In most cases, TSA can be added to an existing assay using the current primary antibody or probe and maintaining the existing detection label and method.
In this webinar, you will:
- Learn the principles of Tyramide Signal Amplification (TSA) and how to apply it to your assay
- Get tips for optimization from experts researchers who have incorporated TSA into their routine assays
- Understand how to multiplex with TSA
- Ask the experts your questions live
Sponsored By PerkinElmer
Moderator
Patrick C.H. Lo, Ph.D.
Associate Editor,
BioTechniques
Speaker
Cláudia Gaspar
Post-doctoral Fellow,
Developmental Biology Unit
Instituto de Medicina Molecular
Speaker
Wanda Wang
Research Specialist,
Division of Neuropathology
University of Pittsburgh School of Medicine
Speaker
Jeffrey Killian, Ph.D.
Director, Bio-discovery
Global Technical Support
PerkinElmer, Inc.
Free Web Event
Available on Demand
Free Web Event
Available on Demand
(This *.mov file works best with Quicktime media player, which is available from Apple as a free download.)
Live cell imaging has become a cornerstone of modern cell biology, as well as a technique routinely employed in other fields such as developmental biology, stem cell biology and neuroscience. In this webinar, you will learn about the effective use of live cell imaging for a growing number of applications. Presentations on the latest microscopy equipment and imaging components to enable live cell imaging, the most effective ways to maintain cells in a healthy state during live cell imaging, and the use of advanced imaging techniques such as FRET and FRAP will enable both novice and advanced users to better apply live cell imaging to their research efforts.
In this webinar, you will:
- Learn about the latest advances in microscopy and imaging components with a perspective on how to achieve optimal imaging results
- Learn the most effective ways to keep cells healthy and alive during live cell imaging
- Discover some of the latest applications and techniques using laser-scanning live cell confocal microscopes
- Be able to ask your live cell imaging questions to a panel of experts
Sponsored By Leica Microsystems:
Moderator:
Patrick C.H. Lo, Ph.D.
Associate Editor,
BioTechniques
Speakers:
Simon C. Watkins, Ph.D., FRCPath
Professor and Vice Chair, Department of Cell Biology and Physiology; Professor of Immunology; and Director, Center for Biologic Imaging, University of Pittsburgh
Richard W. Cole
Director, Advanced Light Microscopy and
Image Analysis Core, Wadsworth Center,
New York State Department of Health and Research Assistant Professor, Department of Biomedical Sciences, School of Public Health, State University of New York
Ivan Robert Nabi, Ph.D.
Professor and Director of Research,
Department of Cellular and Physiological Sciences and Director for the LSI Imaging
Facility, Life Sciences Institute, University of
British Columbia
Free Web Event
Available on Demand