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Video Date: February 25 2013 Run Time:
Confocal and Two-photon Microscopy Methods for Imaging the Brain
Wednesday, May 22, 2013
1 PM EDT / 10 AM PDT / 6 PM UK
A FREE Web Event
Registration Open Now
A deeper understanding of brain function requires visualization of the complex architecture of neurons and their connections. With the ability to peer deeper into samples than conventional light microscopy approaches, confocal and two-photon microscopy are proving to be indispensable tools for such brain mapping studies. In this special webinar, two researchers working at the cutting-edge of brain imaging will discuss their applications of the latest confocal and two-photon microscopy techniques. From fluorescent labeling and imaging of neural circuits using the Brainbow system to in vivo imaging of brain structure, function, and blood flow, this webinar will give researchers a deeper appreciation of the potential for new microscopy methods to unlock the secrets of the cellular world. Unique insights into sample handling and processing of multicolor images will also be presented, and attendees will have the opportunity to ask questions.
Moderator:
Patrick C.H. Lo, Ph.D.
Senior Editor, BioTechniques
Speakers:
Dawen Cai, Ph.D.
Assistant Research Scientist, Department of Physics,
University of Michigan
Ian R. Winship, Ph.D.
Assistant Professor, Neurochemical Research Unit,
Department of Psychiatry,
University of Alberta
Sponsored by:
A Free Web Event
Workshop on Recent Advances and Applications of Laser Capture Microdissection
A Free Web Event
Available on Demand Now
Since its introduction in 1996, laser capture microdissection (LCM) has emerged as an important technique for isolation of specific subsets of cells from complex tissues or other biological samples. Cellular material isolated by LCM is often used in downstream applications including PCR, microarray analysis, and sequencing. Several recent advances in LCM approaches have enhanced and expanded the range of these applications even further. In this web-based workshop, an overview of LCM will be provided along with descriptions and discussions of several recent LCM technology advances.
Attendees will:
- Learn about the development and evolution of LCM approaches, including common applications and troubleshooting tips
- Gain valuable insights into new developments in LCM technology, including the development of expression microdissection techniques
- Engage in a discussion/question and answer session with a panel of LCM experts
Moderator:
Patrick C.H. Lo, Ph.D.
Associate Editor, BioTechniques
Speakers:
Robert Bonner, Ph.D.
Head, Section of Medical Biophysics. National Institute of Child Health and Human Development, National Institutes of Health
Michael Tangrea, Ph.D.
Staff Scientist, Pathogenetics Unit. National Cancer Institute, National Institutes of Health
Jason Hipp, MD., Ph.D.
Rabson fellow, Laboratory of Pathology, National Cancer Institute, National Institutes of Health
Sponsored by:
A Free Web Event
Available on Demand Now
Digital Pathology: Expanding the Microscope Slide Box
A Free Web Event
Available On Demand Now
Understanding human disease progression at the cellular level often requires examination of tissue sections on slides. With its ability to generate and store images of microscope slides and thereby allow scientists to examine specific anatomical locations and landmarks quickly and efficiently, digital pathology has made tremendous inroads in both academic and clinical laboratory settings. In this special webinar, attendees will have a unique opportunity to learn more about: (i) digital pathology applications, (ii) instrumentation and technology (both in a low-throughput and high-throughput format), and (iii) analysis tools and data storage solutions. Be a part of this special web event, and learn more about how digital pathology is enabling students, research scientists, and clinical pathologists gain a more comprehensive understanding of anatomical pathology and human disease.
Attendees will:
- Learn about the implementation of digital pathology workflows in academic and clinical settings
- Hear about the latest approaches in digital pathology image analysis
- Find out about the challenges of data storage and security when establishing a digital pathology workflow
- Be able to ask questions to a panel of experts
Moderator:
Patrick C.H. Lo, Ph.D.
Associate Editor, BioTechniques
Speakers:
John Vallone, M.D., Ph.D.
Associate Professor of Clinical Medicine, Keck School of Medicine, University of Southern California
Cynthia Loomis, M.D., Ph.D.
Director, Experimental Pathology Shared Resource, NYU School of Medicine
Sponsored by:
A Free Web Event
Available On Demand Now
Obtaining Multiparametric, Real-Time Measurements in Industrial Bioprocessing Using Automated Flow Cytometry
A Free Web Event
Monitoring biomass is crucial when optimizing growth conditions. Compared to traditional biomass monitoring methods, flow cytometry is rapid, accurate, and enables multiparametric analysis of heterogeneous populations with single-cell resolution. With development of automated cell sampling and preparation devices, flow cytometry can analyze biomass at regular intervals to precisely control and optimize cell cultures in bioreactors.
In this webinar, we will discuss how the development of an interface between bioreactors and flow cytometry analyzers makes possible automated, real-time bioprocess control. We will demonstrate how automated flow cytometry provides highly detailed data on the dynamics of cell populations, enabling an unprecedented degree of bioreactor monitoring resolution and control.
Finally, we will discuss how automated flow cytometry can be used to maintain a culture in a steady-state condition of low cell density, called a cytostat, in which the selection condition imposed on the culture is precisely defined by the feed medium. With growth conditions matched to an optimal isolation strategy, faster growing, robust mutants are easily detected. Continuous cultivation in cytostat mode offers a powerful method for the rapid development and evolution of desired strains.
In this webinar you will learn:
- How automated flow cytometry enables real-time multiparametric monitoring and control of cell cultures.
- How to use automated flow cytometry to optimize development and evolution of desired strains.
Moderator:
S. Anne Montgomery
Editor in Chief, BioProcess International
Speakers:
Christian Lavarreda
Product Manager for Pharma and Biotech Equipment and Services, MSP Corporation
Friedrich Srienc
Professor of Chemical Engineering and Materials Science, The University of Minnesota Institute of Biotechnology
Dr. Maria Dinkelmann
Senior Marketing Applications Specialist, Personal Flow Cytometry Platforms / BD Biosciences
Sponsored by:
