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Checking Transfection Efficiency

02/20/2013
Kristie Nybo, Ph.D.

Is there a simple way to check transfection efficiency by viewing GFP that saves time?


The BioTechniques Molecular Biology Forums brings bench scientists together in an online community to ask for and share advice regarding laboratory problems. This week’s highlighted question comes from the Microscopy and Imaging Techniques forum.

Is there a simple way to check transfection efficiency by viewing GFP that saves time? Source: BioTechniques




Q: I often need to quickly check transfection efficiency by viewing GFP, but in order to use the microscope, it takes about 10 minutes to start up the system, warm up the mercury burner, switch to the correct fluorescence cubes, etc. before I can focus and look at my samples. Is there a simplified way to do this that would save time?

A: There was a cool Benchmarks paper in BioTechniques a few years ago showing that you could use an LED source to visualize GFP fluorescence: http://www.biotechniques.com/Biotechniq ... 45372.html. An LED light source wouldn't need…

A: This company sells a fluorescent attachment with which…

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