BioTechniques Molecular Biology Forums brings bench scientists together in an online community to ask for and share advice regarding laboratory problems. This week’s highlighted question comes from the Real-Time qPCR/qRT-PCR Methods forum.
Q: I would like exchange ions in my DNA using Slide-A-Lyzer mini pots for the dialysis. First, does anyone have some literature hints?
Secondly, I know dialysis can lead to dilution and sample loss, so how much higher should my starting concentrationbe ?
And finally, how can I calculate the optimum time for dialysis, the right temperature, please?
A: If you are not near the size cutoff, there shouldn't be much loss, except the bit of volume stuck in...
A: For reference information on dialysis (for protein research), you can go to this website...
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