to BioTechniques free email alert service to receive content updates.
DNA Dialysis Questions

01/22/2013
BioTechniques staff

I know my target DNA concentration, but dialysis can lead to dilution and sample loss. How much higher should my starting concentration be?


BioTechniques Molecular Biology Forums brings bench scientists together in an online community to ask for and share advice regarding laboratory problems. This week’s highlighted question comes from the Real-Time qPCR/qRT-PCR Methods forum.

A forum member has some questions about using dialysis for ion exchange. Source: Wikipedia






Q: I would like exchange ions in my DNA using Slide-A-Lyzer mini pots for the dialysis. First, does anyone have some literature hints?

Secondly, I know dialysis can lead to dilution and sample loss, so how much higher should my starting concentrationbe ?

And finally, how can I calculate the optimum time for dialysis, the right temperature, please?

A: If you are not near the size cutoff, there shouldn't be much loss, except the bit of volume stuck in...

A: For reference information on dialysis (for protein research), you can go to this website...

To read answers to this question or to ask or advise others on this or any molecular biology topic, visit BioTechniques Molecular Biology Forums.

Keywords:  troubleshooting forum