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Drug Sensitivity | Troubleshooting Forum

05/22/2013
Kristie Nybo, Ph.D.

I want to determine if my target gene is associated with drug resistance in cancer cells. How can I pursue the role of this gene in drug sensitivity?


The BioTechniques Molecular Biology Forum brings scientists together in an online community to ask for and share advice regarding laboratory problems. This week’s highlighted question comes from the Student Questions forum.

Cancer cells in culture from human connective tissue, illuminated by darkfield amplified contrast, at a magnification of 500x. Source: National Cancer Institute




Q: I want to determine if my target gene is associated with drug resistance in cancer cells. I first used RNAi to reduce expression and found that the cells became more sensitive to the drug, but overexpression of the target gene produced no effect. How can I pursue the role of this gene in drug sensitivity?

A: You might try using non-cancer cells for studies of over-expression. Transfection is not very efficient, so you may need to use…

A: Try screening various cancer cell lines to find one with…

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