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ELISA | Troubleshooting Forum

01/29/2013
Kristie Nybo, Ph.D.

Is it possible to attach a small oligonucleotide labeled with Cy5 to a microplate to serve as an antigen for ELISA analysis


BioTechniques Molecular Biology Forum brings bench scientists together in an online community to ask for and share advice regarding laboratory problems. This week’s highlighted question comes from the Immunology and Immunochemistry forum.

Q: Is it possible to attach a small oligonucleotide labeled with Cy5 to a microplate to serve as an antigen for ELISA analysis?

Is it possible to attach a small oligonucleotide labeled with Cy5 to a microplate to serve as an antigen for ELISA analysis? Source: Jeffrey M. Vinocur, Wikimedia




A: I’m not sure if you would get an antibody response to a DNA fragment that small. DNA elements that generate immune responses seem to be…

A: Anti-Cy5 antibodies are available from a number of companies. You might try purchasing one before generating your own. As for attaching an oligo to a solid surface, please read...

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