The eels have it—at least when it comes to understanding why brain cells die after a spinal cord injury.
Using lamprey eels, Petromyzon marinus, scientists have shown that after injury, the brain cells that deteriorate and die tend to build up more of the protein synuclein compared to the cells that survive. This protein accumulation is similar to what occurs during neurodegenerative diseases such as Parkinson’s disease and could be instrumental in the development of treatments.
She and fellow UT-Austin neuroscientist David Busch identified the link between synuclein build-up and cell death using the "subset of very large, identifiable neurons," called reticulospinal, or RS neurons in lamprey eels. Because these giant neurons are located in similar spots in every lamprey brain, scientists can easily study them in a large number of animals.
While many of the lamprey eel’s RS neurons are "reproducibly good" at surviving an injury and regenerating their axons, others die. "By using the lamprey model, we can examine the molecular events that are happening in vivo in 'good survivors' versus 'poor survivors' and how these molecular events impact the regeneration process," Morgan said.
In the experiment, Morgan severed a group of eels' spinal cords at their fifth gill. Using Nissl and Fluoro-Jade C staining, the scientists monitored these RS neurons after injury. After week one, many of the neurons remained healthy, but by week three they began to degenerate. After week 11, 30% of all of the RS neurons in injured lampreys had deteriorated.
Next, the researchers used a synuclein antibody and immunofluorescence to measure the build up of synuclein in the RS neurons. The results showed that the greatest protein build-up was in the "poor survivor" neurons.
"The link between cell-specific synuclein accumulation and neurodegeneration after spinal cord injury had not been made before this study," Morgan said. Because synuclein accumulation is a bioindicator of imminent neuronal death in Parkinson’s disease, strategies to diagnose and treat Parkinson’s could potentially be effective in patients with spinal cord injuries.
Next, Morgan will attempt to disrupt synuclein accumulation post injury to further explore its relationship to neurodegeneration. "Spinal cord injury will be an area of research and medicine where we will continue to see significant progress over the next decade," said Morgan.
- Busch, D. and Morgan, J. (2012). "Synuclein Accumulation Is Associated With Cell-Specific Neuronal Death After Spinal Cord Injury." The Journal of Comparative Neurology. 520:1751–1771.