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Inclusion Bodies | Troubleshooting Forum

11/12/2012
Kristie Nybo, Ph.d.

Can I extract native-like secondary structured proteins from inclusion bodies using a low concentration of urea?


BioTechniques Molecular Biology Forum brings bench scientists together in an online community to ask for and share advice regarding laboratory problems. This week’s highlighted question comes from the Protein Methods forum.

Q: I am working with protein in inclusion bodies in E. coli. I have had difficulty with protein refolding, but read that I could extract native-like secondary structured proteins from inclusion bodies using a low concentration of urea. Does anyone have experience with this or know of a good protocol?





A: There are several ways to coax a protein into a soluble fraction: temperature, IPTG concentrations, etc. An approach you might have overlooked is…

A: Sarkosyl works well for solubilizing inclusion bodies. A good reference can be found…

To read answers to this question or to ask or advise others on this or any molecular biology topic, visit the Protein Methods forum.

Keywords:  troubleshooting fourm