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Laser Capture Microdissection | Troubleshooting Forum
10/09/2012
Kristie Nybo, Ph.D.
What can I do to improve the yield and quality of the RNA?
The BioTechniques Molecular Biology Forum brings bench scientists together in an online community to ask for and share advice regarding laboratory problems. This week’s highlighted question comes from the Microscopy and Imaging Techniques forum.
A: For isolating RNA, you need to use a membrane slide if you are using a 2 laser system. I would recommend that the samples…
A: Remember not to rely on oligo dT primers for the reverse transcription step if you get enough RNA out of your tissue because formaldehyde modifies the adenine in poly-A tails and therefore…
To read answers to this question or to ask or advise others on this or any molecular biology topic, visit BioTechniques Molecular Biology Forum.
Keywords:
troubleshooting
RNA
laser capture microdissection
