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Metatranscriptomics | Troubleshooting Forum

03/13/2013
Kristie Nybo, Ph.D.

I'm having some trouble using Trizol to extract total RNA from a complex microbial community (rumen contents). How can I improve my results?


The BioTechniques Molecular Biology Forum brings bench scientists together in an online community to ask for and share advice regarding laboratory problems. This week’s highlighted question comes from the RNA Methods forum.

The BioTechniques Molecular Biology Forum brings bench scientists together in an online community to ask for and share advice regarding laboratory problems.




Q: I'm having some trouble using Trizol to extract total RNA from a complex microbial community (rumen contents). The samples will be used for Illumina sequencing for metatranscriptomics. Some samples return clean RNA without gDNA contamination, while other samples return no rRNA or have a lot of gDNA contamination. How can I improve my results?

A: The composition of cellular RNA depends a lot on the growth phase from which the cells were harvested. Try to passage…

A: Consider the influence of the guanidinium thiocyanate in the Trizol on your expected result. You might try to do a simple…

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