Mitochondrial genome sequencing
Kristie Nybo, Ph.D.
Can anyone suggest a good protocol for isolating mitochondria from whole insects?
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Q: Can anyone suggest a good protocol for isolating mitochondria from
whole insects? I would like to sequence the mitogenome of an insect.
You may be able to isolate the mitochondrial fraction from a cell
line, but this will require optimization and waste a lot of cells. My
colleagues from a previous lab used to do PCR after extracting…
A: I would recommend you use Phusion and perform a long PCR. I have
amplified plasmids up to.…
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