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Mitochondrial genome sequencing

09/19/2012
Kristie Nybo, Ph.D.

Can anyone suggest a good protocol for isolating mitochondria from whole insects?


The BioTechniques Molecular Biology Forum brings bench scientists together in an online community to ask for and share advice regarding laboratory problems. This week’s highlighted question comes from the DNA and General PCR Methods forum.

Q: Can anyone suggest a good protocol for isolating mitochondria from whole insects? I would like to sequence the mitogenome of an insect.

Can anyone suggest a good protocol for isolating mitochondria from whole insects? Source: Gerald J. Lenhard, Louisiana State University.





A: You may be able to isolate the mitochondrial fraction from a cell line, but this will require optimization and waste a lot of cells. My colleagues from a previous lab used to do PCR after extracting…

A: I would recommend you use Phusion and perform a long PCR. I have amplified plasmids up to.…

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