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RNA Extraction from Plasma

09/25/2012
Kristie Nybo

What is the best approach for collecting RNA only from plasma?


The BioTechniques Molecular Biology Forum brings bench scientists together in an online community to ask for and share advice regarding laboratory problems. This week’s highlighted question comes from the RNA Methods forum.

Q: I want to extract RNA from plasma beginning with blood samples collected in Paxgene blood RNA tubes. The kit accompanying the tubes is for extracting whole blood. What is the best approach for collecting RNA only from plasma?

A: You could pellet or concentrate the cells in the RNA preservative before RNA extraction. The preservative protocol might mention…

A: You might try Tri-Reagent-LS (or Trizol LS) instead of…

Schematic of the mouse cell isolation, RNA extraction, hybridization to Affymetrix 430.2 microarray chips, and statistical analysis. Source: The Annals of Thoracic Surgery

To read answers to this question or to ask or advise others on this or any molecular biology topic, visit BioTechniques Molecular Biology Forum.

Keywords:  microscopy