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RNA Extraction from Plasma

12/19/2012
Kristie Nybo, Ph.D.

Can I just spin the samples I have and use Trizol to extract RNA from the plasma?


The BioTechniques Molecular Biology Forum brings bench scientists together in an online community to ask for and share advice regarding laboratory problems. This week’s highlighted question comes from the RNA Methods forum.

Q: I would like to extract RNA from plasma, beginning with blood collected in Paxgene RNA tubes. The kit is for extracting intracellular RNA from whole blood. Can I just spin the samples I have and use Trizol to extract RNA from the plasma?





A: Since you have a liquid sample, you may need Tri-Reagent-LS…

A: You might try to pellet or concentrate the cells in the RNA preservative before…

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