BioTechniques Molecular Biology Forum brings bench scientists together in an online community to ask for and share advice regarding laboratory problems. This week’s highlighted question comes from the Real-Time qPCR/qRT-PCR Methods forum.
Q: I would like to quantify the expression of some genes using real-time PCR. I have successful primers for standard PCR; will these work for real-time PCR as well? How do I need to modify the primers for use in real-time PCR?
A: I would suggest using primers designed for products up to 300bp in size, which will allow for better resolution…
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