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Real-Time PCR Primers

01/17/2013
Kristie Nybo, Ph.D.

I have successful primers for standard PCR; will these work for real-time PCR as well?


BioTechniques Molecular Biology Forum brings bench scientists together in an online community to ask for and share advice regarding laboratory problems. This week’s highlighted question comes from the Real-Time qPCR/qRT-PCR Methods forum.

Q: I would like to quantify the expression of some genes using real-time PCR. I have successful primers for standard PCR; will these work for real-time PCR as well? How do I need to modify the primers for use in real-time PCR?






A: If you are going to use SYBR Green, you need to keep in mind…

A: I would suggest using primers designed for products up to 300bp in size, which will allow for better resolution…

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Keywords:  troubleshooting pcr