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Taqman Probes | Troubleshooting Forum

02/05/2013
Kristie Nybo, Ph.D.

Can I use the FISH probe as my TaqMan probe if I design forward and reverse primers on either side of it?


The BioTechniques Molecular Biology Forum brings bench scientists together in an online community to ask for and share advice regarding laboratory problems. This week’s highlighted question comes from the Real-Time qPCR/qRT-PCR Methods forum.

The BioTechniques Molecular Biology Forum brings bench scientists together in an online community to ask for and share advice regarding laboratory problems.




Q: I want to quantify bacteria using Taqman RT-PCR. I previously detected the bacteria by FISH using a probe that targets the 16S RNA. Can I use the FISH probe as my TaqMan probe if I design forward and reverse primers on either side of it?

A: The guidelines for qPCR probes are different from those for FISH probes. You will need to…

A: There may be ready-made probe and primer sets for your purpose. Try looking for them in...

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