Top 10 Peer-Reviewed Research Articles | 2012 Year in Review
Andrew S. Wiecek
What were the most viewed peer-reviewed methods published in BioTechniques in 2012? Find out..
In 2012, BioTechniques published a wide range of methods, including articles
on the cell-free expression of proteins and library-free DNA sequencing. The
goal of every paper that we publish is to move life science research forward
through innovative technical advances that leverage the latest tools and
Out of all the methods that we published in 2012, the one that received the
most views on our website was a method for the quantitative analysis of
microRNA expression by in situ hybridization (ISH). What is remarkable about
this technique is that researchers can now assessment the miRNAs expressed
of epithelial tumors directly without the need to extract total RNA from
these cells. The method was developed in the lab of Yale University
pathologist David L. Rimm.
As always, we encourage you to sign up for our monthly
Table of Content alerts and Rapid
Dispatches alerts to ensure that you don’t miss a new method that could
improve your experiments.
And without further ado, here is the complete top 10 peer-reviewed research
articles that BioTechniques published in 2012 as ranked by pageviews on our
analysis of microRNAs in tissue microarrays by in situ hybridization,”
by Hanna et al.
2. “Length and
GC-biases during sequencing library amplification: a comparison of various
polymerase-buffer systems with ancient and modern DNA sequencing libraries,”
by Dabney and Meyer.
3. “Automated high
multiplex qPCR platform for simultaneous detection and quantification of
multiple nucleic acid targets,” by Hlousek et al.
of cofilin-actin and Ras-Raf interactions by bimolecular fluorescence
complementation assays using a new pair of split Venus fragments,” by
Ohashi et al.
5. “U-2012: An
improved Lowry protein assay, insensitive to sample color, offering reagent
stability and enhanced sensitivity,” by Upreti et al.
three-dimensional reconstruction of a whole yeast cell using focused-ion
beam scanning electron microscopy,” by Wei et al.
of artifactual microarray probe signals constantly present in multiple
sample types,” by Mao et al.
multiple target detection in real-time loop-mediated isothermal amplification,”
by Tanner et al.
9. “Technique for
strand-specific gene-expression analysis and monitoring of
primer-independent cDNA synthesis in reverse transcription,” by Feng et
sequencing of small genomes on the Pacific Biosciences RS without library
preparation,” by Coupland et al.