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Top 10 Peer-Reviewed Research Articles | 2012 Year in Review

12/21/2012
Andrew S. Wiecek

What were the most viewed peer-reviewed methods published in BioTechniques in 2012? Find out..


In 2012, BioTechniques published a wide range of methods, including articles on the cell-free expression of proteins and library-free DNA sequencing. The goal of every paper that we publish is to move life science research forward through innovative technical advances that leverage the latest tools and technology.

In 2012, BioTechniques published a wide range of methods, including articles on the cell-free expression of proteins and library-free DNA sequencing.





Out of all the methods that we published in 2012, the one that received the most views on our website was a method for the quantitative analysis of microRNA expression by in situ hybridization (ISH). What is remarkable about this technique is that researchers can now assessment the miRNAs expressed of epithelial tumors directly without the need to extract total RNA from these cells. The method was developed in the lab of Yale University pathologist David L. Rimm.

As always, we encourage you to sign up for our monthly Table of Content alerts and Rapid Dispatches alerts to ensure that you don’t miss a new method that could improve your experiments.

And without further ado, here is the complete top 10 peer-reviewed research articles that BioTechniques published in 2012 as ranked by pageviews on our website:

1. “Quantitative analysis of microRNAs in tissue microarrays by in situ hybridization,” by Hanna et al.

2. “Length and GC-biases during sequencing library amplification: a comparison of various polymerase-buffer systems with ancient and modern DNA sequencing libraries,” by Dabney and Meyer.

3. “Automated high multiplex qPCR platform for simultaneous detection and quantification of multiple nucleic acid targets,” by Hlousek et al.

4. “Visualization of cofilin-actin and Ras-Raf interactions by bimolecular fluorescence complementation assays using a new pair of split Venus fragments,” by Ohashi et al.

5. “U-2012: An improved Lowry protein assay, insensitive to sample color, offering reagent stability and enhanced sensitivity,” by Upreti et al.

6. “High-resolution three-dimensional reconstruction of a whole yeast cell using focused-ion beam scanning electron microscopy,” by Wei et al.

7. “Identification of artifactual microarray probe signals constantly present in multiple sample types,” by Mao et al.

8. “Simultaneous multiple target detection in real-time loop-mediated isothermal amplification,” by Tanner et al.

9. “Technique for strand-specific gene-expression analysis and monitoring of primer-independent cDNA synthesis in reverse transcription,” by Feng et al.

10. “Direct sequencing of small genomes on the Pacific Biosciences RS without library preparation,” by Coupland et al.

Keywords:  2012 year in review