The top peer-reviewed research article published in BioTechniques in 2011, as determined by the number of pageviews on our web site, is a review of the technical considerations when using fluorescent probes to measure mitochondrial membrane potential. In this article, Seth W. Perry and colleagues from the University of Rochester School of Medicine and Dentistry outline the strengths and weaknesses of common dyes, as well as best practices in the approach.
membrane potential probes and the proton gradient: a practical usage
Perry et al. provide an overview of some of the important technical considerations, controls, and parallel complementary assays that can be used to help ensure appropriate interpretation of results, thus providing a practical usage guide for monitoring mitochondrial membrane potentials with cationic probes.
fluorescent proteins: from chromophore formation to novel intracellular
Here, Stepanenko et al. review our current knowledge of blue, green, and red chromophore formation in permanently emitting FPs, photoactivatable FPs, and fluorescent timers.
DNA amplification in a capillary tube by natural convection with a
single isothermal heater.”
Chou et al. describe a simple platform for rapid DNA amplification using convection.
microstreaming increases the efficiency of reverse transcription
reactions comprising single-cell quantities of RNA.”
In this study, Boon et al. tested the capacity for a novel acoustic microstreaming method (“micromixing”), which stirs fluid at microliter scales, to improve cDNA yields from reverse transcription (RT) reactions comprising single-cell quantities of RNA.
of genomic DNA from yeasts for PCR-based applications.”
Lõoke et al. have developed a quick and low-cost genomic DNA extraction protocol from yeast cells for PCR-based applications.
for improved Illumina sequencing library preparation using NuGEN
Ovation RNA-Seq System.”
In this study, Head et al. tested the NuGEN Ovation RNA-Seq System for library preparation, followed by next-generation sequencing on an Illumina GAIIx.
sequencing of core STR loci for forensic genetic investigations using
the Roche Genome Sequencer FLX platform.”
Here, Fordyce et al. present an STR profiling method based on the use of the Roche Genome Sequencer (GS) FLX to simultaneously sequence multiple core STR loci.