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Transformation | Troubleshooting Forum

11/20/2012
Kristie Nybo, Ph.D.

The growth rate of the resulting colonies was very slow in LB broth and on plates containing ampicillin. How can I improve this?


BioTechniques Molecular Biology Forum brings bench scientists together in an online community to ask for and share advice regarding laboratory problems. This week’s highlighted question comes from the DNA and General PCR Methods forum.

Q: I transformed a recombinant plasmid into B. subtilis and got many clones, but the growth rate of the resulting colonies was very slow in LB broth and on plates containing ampicillin. How can I improve this?





A: It may be that your gene is toxic to the cells. Did you transform…

A: Is the growth rate too slow to produce enough plasmid for purification? You might try growing them at…

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