BioTechniques Molecular Biology Forum brings bench scientists together in an online community to ask for and share advice regarding laboratory problems. This week’s highlighted question comes from the Protein Methods forum.
Q: I'm blotting to detect a 150kDa membrane channel. I get a reproducible, concentration-dependent band that runs at about 110kDa, but no band at 150kDa. This band vanishes when I pre-incubate, so I'm sure it's the primary antibody rather than the secondary that's reacting.
A: Try epitope tagging the protein and expressing it in a human cell line…
A: It could be a regulatory splice variant. Try RT-PCR for…
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