Open any life science journal, and you will likely see article study that takes advantage of next-generation sequencing (NGS). Clearly, nucleic acid sequencing remains a critical application for life scientists, but the technology is also becoming an important source of inspiration for methods developers as they develop new techniques and instrument refinements that will further expand the power of NGS. The articles below include techniques for modifying instruments to improve throughput, enhanced protocols for creating libraries and new applications for NGS technology. But they all have a common element—each article further increases the value of DNA sequencing applications in the lab.
1. “Simultaneous digital quantification and fluorescence-based size characterization of massively parallel sequencing libraries” by Laurie et al.
2. “A high-plex PCR approach for massively parallel sequencing" Nyguyen-Dumont et al.
3. “Lane-by-lane sequencing using Illumina’s Genome Analyzer II” by Gravina et al.
4. “Dual primer emulsion PCR for next generation DNA sequencing” by Xu et al.
5. “Direct sequencing of small genomes on the Pacific Biosciences RS without library preparation” by Coupland et al.
6. “Direct PCR amplification and sequencing of specimens’ DNA from preservative ethanol” by Shokralla et al.
7. “Improving sequencing quality from PCR products containing long mononucleotide repeats” by Fazekas et al.
8. “Length and GC-biases during sequencing library amplification: A comparison of various polymerase-buffer systems with ancient and modern DNA sequencing libraries” by Dabney et al.
9. “Profiling the HeLa S3 transcriptome using randomly primed cDNA and massively parallel short-read sequencing” by Morin et al.
10. “Creation and application of immortalized bait libraries for targeted enrichment and next-generation sequencing" Querfurth et al.