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geNorm

05/11/2011
Kristie Nybo, Ph.D.

When using geNorm, what is the recommended M value cut-off for heterogeneous samples?

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The BioTechniques Molecular Biology Forums brings bench scientists together in an online community to ask for and share advice regarding laboratory problems. This week’s highlighted question comes from the Real Time qPCR/qRT-PCR Methods forum.

Q: When using geNorm, what is the recommended M value cut-off for heterogeneous samples? The literature recommends using the geometric mean of 3 genes for normalization, but if the M value is < 0.2, will one suffice?

A: If you can unequivocally prove that you get the same Cq value for your reference gene in all samples loaded equally into all reactions, and can additionally demonstrate that this Cq…

A: Data normalization can be carried out against an endogenous unregulated reference gene transcript or against total cellular DNA or RNA content, but…

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