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2010 Protocol Guide
BioTechniques' Protocol Guide 2010 is a collection of detailed, optimized procedures/protocols for use in the lab. With BioTechniques' Protocol Guide, you have access to detailed product information in a downloadable PDF format-which can be easily added to a notebook for ready access. Each protocol is accompanied by a link to the company web site and an e-mail link to contact the company directly.
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Antibody-based Technologies
Immunostaining
Immunostaining protocols for flow cytometric analysis of adherent cells
Ricardo B. deMedeiros, Ph.D. and Joy Aho, Ph.D.
R&D Systems, Inc.
doi10.2144/000113280
Immunofluorescence
Biomarker Validation
Cell Culture
Cytometry
Flow Cytometry
Analysis of mitochondrial membrane potential
Accuri Cytometers doi10.2144/000113278
DNA/RNA Isolation and Purification
From Soil/Environmental Samples
Extraction of inhibitor-free metagenomic DNA from soil
Zymo Research Corporation doi10.2144/000113292
From FFPE Archival Tissues
Isolation of DNA from FFPE samples without paraffin removal
Suzanne Kennedy, Ph.D., Director of R&D
Mo Bio Laboratories, Inc.
doi10.2144/000113291
Environmental Microbiology Extraction
Isolation of DNA and RNA from soil using two different methods optimized with Inhibitor Removal Technology® (IRT)
Suzanne Kennedy, Ph.D., Director of R&D
Mo Bio Laboratories, Inc.
doi10.2144/000113290
Electroporation
Optimization of nucleic acid delivery into immune cell lines using Ingenio™ Electroporation Solution
Anjana N. Bhattacharya, Ph.D., and B. Gopalakrishnan, Ph.D.
Mirus Bio LLC
doi10.2144/000113279
Epigenetics
Methylation Detection
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