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2010 Protocol Guide
BioTechniques' Protocol Guide 2010 is a collection of detailed, optimized procedures/protocols for use in the lab. With BioTechniques' Protocol Guide, you have access to detailed product information in a downloadable PDF format-which can be easily added to a notebook for ready access. Each protocol is accompanied by a link to the company web site and an e-mail link to contact the company directly.
2010 Protocol Guide
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Gene Transfer
Transfection
Lentiviral Production Using X-tremeGENE HP Transfection Reagent
Roche Applied Science (Subsidiary of Roche)
Efficient transfection of mammalian primary and stem cells using the Neon™ Transfection System
Invitrogen Corporation, part of Life Technologies doi10.2144/000113288
Labeling and Detection
Enhanced detection sensitivity of luciferase reporter gene assays
PerkinElmer doi10.2144/000113284
MicroRNA
MicroRNA Labeling
A simple and accurate method for labeling plant and animal microRNAs
Mirus Bio LLC doi10.2144/000113306
Microscopy
Multi-Label Techniques for Colocalization Studies
User protocol for multi-color immunohistochemistry staining in intact tissue
Chris van der Loos, Ph.D.
Cambridge Research & Instrumentation, Inc. (CRi) doi10.2144/000113281
PCR
HRM (High Resolution Melt)
Gradient optimization of a high-resolution melt (HRM) assay for targeted cancer therapy screening using SsoFast™ EvaGreen® supermix and the CFX96™ real-time PCR detection system
Bio-Rad Laboratories doi10.2144/000113287
Peer-Reviewed Protocols
Practical DGS protocol
Yong-Chul Jung1, Jia Xu2, Jun Chen1, Yeong C. Kim1, David J. Winchester3, and San Ming Wang1
doi10.2144/000113307
Cryopresevation of mouse embryos with a vitrification spatula
Wai Hung Tsang and King L. Chow
doi10.2144/000113258
Preparation of formalin-fixed paraffin-embedded (FFPE) tissues for RNA extraction
Kim M. Linton1,5*, Yvonne Hey2*, Sian Dibben2, Crispin J. Miller3, Anthony J. Freemont4, John A. Radford1,5, and Stuart D. Pepper2
doi10.2144/000113261
Extraction of total RNA from fresh/frozen tissue (FT)
Kim M. Linton1,5*, Yvonne Hey2*, Sian Dibben2, Crispin J. Miller3, Anthony J. Freemont4, John A. Radford1,5, and Stuart D. Pepper2
doi10.2144/000113260
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Submit Your Protocols
BioTechniques is always looking for author-submitted protocols related to peer-reviewed papers, so submit your protocols today to our editorial team. Suppliers or advertisers interested in publishing their protocols should also submit.
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