2010 Protocol Guide
BioTechniques' Protocol Guide 2010 is a collection of detailed, optimized procedures/protocols for use in the lab. With BioTechniques' Protocol Guide, you have access to detailed product information in a downloadable PDF format-which can be easily added to a notebook for ready access. Each protocol is accompanied by a link to the company web site and an e-mail link to contact the company directly.
2010 Protocol Guide
BioTechniques' Protocol Guide 2010
November 2009

Contents
Antibody-based Technologies Biomarker Validation Cell Culture Cytometry DNA/RNA Isolation and Purification Electroporation Epigenetics Gene Transfer Labeling and Detection MicroRNA Microscopy PCR Peer-Reviewed Protocols Protein Extraction Isolation and Purification RNAi_Protocols
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Antibody-based Technologies
Immunostaining
Immunostaining protocols for flow cytometric analysis of adherent cells
Ricardo B. deMedeiros, Ph.D. and Joy Aho, Ph.D.
R&D Systems, Inc. doi10.2144/000113280
Immunofluorescence
ELISPOT and Dual ELISPOT: a cell-based assay to assess immune response
Abcam plc doi10.2144/00013302
Biomarker Validation
No-wash biomarker assay protocol with improved sensitivity and dynamic range, plus low variability
PerkinElmer doi10.2144/000113283
Cell Culture
Growing CHO cells in a benchtop stirred-tank bioreactor using single-use vessels
New Brunswick Scientific doi10.2144/000113282
Cytometry
Flow Cytometry
Analysis of mitochondrial membrane potential
Accuri Cytometers doi10.2144/000113278
DNA/RNA Isolation and Purification
From Soil/Environmental Samples
Extraction of inhibitor-free metagenomic DNA from soil
Zymo Research Corporation doi10.2144/000113292
From FFPE Archival Tissues
Isolation of DNA from FFPE samples without paraffin removal
Suzanne Kennedy, Ph.D., Director of R&D
Mo Bio Laboratories, Inc. doi10.2144/000113291
Environmental Microbiology Extraction
Isolation of DNA and RNA from soil using two different methods optimized with Inhibitor Removal Technology® (IRT)
Suzanne Kennedy, Ph.D., Director of R&D
Mo Bio Laboratories, Inc. doi10.2144/000113290
Electroporation
Optimization of nucleic acid delivery into immune cell lines using Ingenio™ Electroporation Solution
Anjana N. Bhattacharya, Ph.D., and B. Gopalakrishnan, Ph.D.
Mirus Bio LLC doi10.2144/000113279
Epigenetics
Methylation Detection
Precise quantitation of DNA methylation by HRM analysis using EZ qPCR/HRM PreMix
Zymo Research Corporation doi10.2144/000113293
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