Original
Trans-Blot® Turbo™ Transfer System
DOI: 10.2144/000113791

Abstract

Instructions for Using the Trans-Blot Turbo System

1. Assemble the Blotting Sandwich: After gel electrophoresis, open the transfer pack and assemble the components on the cassette in the order shown below. For best results, use the roller to remove any air trapped between the layers.


(Click to enlarge)


If using a single mini- or midi-format sandwich, it should be placed in the center of the cassette bottom. If using two mini format gels, they should be placed on a midi stack with the foot of each gel toward the center.

2. Insert Cassette into Instrument: Place the lid on the cassette and lock by turning the knob clockwise, using the symbols on the lid as a guide. Slide the cassette into either bay. The upper bay is designated as bay A and the lower bay is designated as bay B. If running two cassettes simultaneously, refer to the table below for acceptable transfer pack combinations.



Acceptable*

Not Acceptable*

* Conditions hold if trays are swapped.

Combination

1

2

1

2

Upper Bay A

1 mini gel

2 mini gels or 1 midi gel

1 mini gel

2 mini gels or 1 midi gel

Lower Bay B

1 mini gel

2 mini gels or 1 midi gel

2 mini gels or 1 midi gel

1 mini gel

3. Start the Transfer: With the cassette inserted into the instrument, press TURBO and select your gel type. Press A:RUN or B:RUN to begin the transfer. LIST will allow you to select from Bio-Rad's optimized protocols (see table below) or a user-defined protocol. NEW will allow you to create and run a new protocol.



Protocol Name

MW, HD

Time, min

2 Mini Gels or 1 Midi Gel

1 Mini Gel

*Also accessed via the TURBO navigation button.

STANDARD SD

Any

30

Up to 1.0 A; 25 V constant

1.5 MM GEL

Any

10

2.5 A constant;up to 25 V

1.3 A constant;up to 25 V

HIGH MW

>150

10

LOW MW

<30

5

MIXED MW*

5–150

7

1 Mini TGX™*

5–150

3

N/A

2.5 A constant; up to 25 V

4. Downstream Processing: At the end of the transfer, RUN COMPLETE will be displayed for the cassette slot.

▪ Pull cassette straight out of slot (Caution: May be warm!) and unlock lid

▪ Disassemble blotting sandwich

▪ Extract membrane, which is ready for downstream processing or storage

▪ Discard remaining transfer pack materials

Key Menu Screens


Refer to the Trans-Blot Turbo system instruction manual for a complete listing of menu screens. (Click to enlarge)


Screen Features


RUN Screen (Click to enlarge)



EDIT Screen (Click to enlarge)


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