Biotechniques Protocols

Biotechniques Protocols http://www.biotechniques.com/protocols/ Protocols en-us ℗ & © Wed Jan 16 19:54:54 GMT 2013 of Informa Pharma. Please refer to the Terms and Conditions of Use at http://www.biotechniques.com rss@biotechniques.com Thu, 01 Nov 2012 17:40:56 +0000 Wed, 16 Jan 2013 19:54:54 +0000 60 Protocols http://www.biotechniques.com/protocols/2012_Protocol_Guide/Development-of-a-Highly-Specific-14-3-3-eta--ELISA-for-Rheumatoid-Arthritis/biotechniques-336730.html Thu, 01 Nov 2012 17:40:56 +0000 http://www.biotechniques.com/protocols/2012_Protocol_Guide/Development-of-a-Highly-Specific-14-3-3-eta--ELISA-for-Rheumatoid-Arthritis/biotechniques-336730.html http://www.biotechniques.com/protocols/Cell_and_Tissue_Analysis/Protocol-for-the-Verification-of-Human-Stem-Cell-Pluripotency/biotechniques-336718.html Wed, 31 Oct 2012 18:53:09 +0000 http://www.biotechniques.com/protocols/Cell_and_Tissue_Analysis/Protocol-for-the-Verification-of-Human-Stem-Cell-Pluripotency/biotechniques-336718.html http://www.biotechniques.com/protocols/2012_Protocol_Guide/Development-and-Research-Validation-of-the-iPLEX----ADME-PGx-Panel-on-the-MassARRAY-System/biotechniques-330915.html Introduction The goals of pharmacogenetic research are to understand and validate drug targets and metabolic pathways, identify optimal dosage, improve drug safety, and understand... Wed, 23 May 2012 19:36:17 +0100 http://www.biotechniques.com/protocols/2012_Protocol_Guide/Development-and-Research-Validation-of-the-iPLEX----ADME-PGx-Panel-on-the-MassARRAY-System/biotechniques-330915.html http://www.biotechniques.com/protocols/2012_Protocol_Guide/Trans-Blot-Turbo-Transfer-System/biotechniques-325107.html Wed, 21 Dec 2011 20:58:44 +0000 http://www.biotechniques.com/protocols/2012_Protocol_Guide/Trans-Blot-Turbo-Transfer-System/biotechniques-325107.html http://www.biotechniques.com/protocols/2012_Protocol_Guide/Practical-SA-cloning-protocol/biotechniques-324735.html Legend ⇒ATTENTION: *HINT: ✋REST<... Wed, 21 Dec 2011 19:12:17 +0000 http://www.biotechniques.com/protocols/2012_Protocol_Guide/Practical-SA-cloning-protocol/biotechniques-324735.html http://www.biotechniques.com/protocols/2012_Protocol_Guide/Rare-allele-enrichment-and-detection-by-allele-specific-PCR-competitive-probe----blocking-and-melting-analysis/biotechniques-324732.html Wed, 21 Dec 2011 20:42:52 +0000 http://www.biotechniques.com/protocols/2012_Protocol_Guide/Rare-allele-enrichment-and-detection-by-allele-specific-PCR-competitive-probe----blocking-and-melting-analysis/biotechniques-324732.html http://www.biotechniques.com/protocols/2012_Protocol_Guide/Quantitation-of-proteins-expressed-on-the-plasma-membrane-using-cold-adapted----proteases/biotechniques-324728.html Wed, 21 Dec 2011 19:17:22 +0000 http://www.biotechniques.com/protocols/2012_Protocol_Guide/Quantitation-of-proteins-expressed-on-the-plasma-membrane-using-cold-adapted----proteases/biotechniques-324728.html http://www.biotechniques.com/protocols/2012_Protocol_Guide/Yeast-genomic-DNA-extraction-with-LiOAc-SDS/biotechniques-324723.html Procedure 1.Pick one yeast colony from the plate or spin down 100-200 µL liquid yeast culture (OD600=0.4). Suspend cells in 100 µL 200 mM LiOAc, 1% SDS solution. 2. Incubate for 5 min at 70°C. 3. Add 300 µL... Tue, 20 Dec 2011 19:29:08 +0000 http://www.biotechniques.com/protocols/2012_Protocol_Guide/Yeast-genomic-DNA-extraction-with-LiOAc-SDS/biotechniques-324723.html http://www.biotechniques.com/protocols/2012_Protocol_Guide/Protocol-for-intelligent-high-content-screening-of-zebrafish-embryos-on-a----standard-widefield-screening-microscope/biotechniques-324719.html Legend ⇒ ATTENTION * HINT ... Tue, 20 Dec 2011 19:37:14 +0000 http://www.biotechniques.com/protocols/2012_Protocol_Guide/Protocol-for-intelligent-high-content-screening-of-zebrafish-embryos-on-a----standard-widefield-screening-microscope/biotechniques-324719.html http://www.biotechniques.com/protocols/2012_Protocol_Guide/Cell-Based-Infrared-ELISAs-Intracellular-Protein-Detection-Without-Lysate----Preparation/biotechniques-324711.html Tue, 20 Dec 2011 19:55:53 +0000 http://www.biotechniques.com/protocols/2012_Protocol_Guide/Cell-Based-Infrared-ELISAs-Intracellular-Protein-Detection-Without-Lysate----Preparation/biotechniques-324711.html http://www.biotechniques.com/protocols/A-rapid-and-highly-sensitive-method-for-somatic-mutation-profiling---using-the-Sequenom-MassARRAY-System/biotechniques-312575.html Introduction <div style="float:right;"><div style=" background-color: #f6f6f6; padding: 3px; margin: 10px; border: 1px solid #ccc; background-color:#f6f6f6; color: #000; text-align: left; width:320px; font-size:90%; font:Arial, Helvetica,... Fri, 11 Mar 2011 19:53:43 +0000 http://www.biotechniques.com/protocols/A-rapid-and-highly-sensitive-method-for-somatic-mutation-profiling---using-the-Sequenom-MassARRAY-System/biotechniques-312575.html http://www.biotechniques.com/protocols/Antibody-based_Technologies/Multi-analyte_Profiling/Proteome-Profiler-antibody-arrays-adapted-for-use-with-the-LI-COR-system/biotechniques-305712.html <div style=" background-color: #f6f6f6; padding: 3px; margin: 10px; border: 1px solid #ccc; background-color:#f6f6f6; color: #000; float: right; text-align: left; width:320px; font-size:90%; font:Arial, Helvetica, sans-serif;... Tue, 09 Nov 2010 18:21:25 +0000 http://www.biotechniques.com/protocols/Antibody-based_Technologies/Multi-analyte_Profiling/Proteome-Profiler-antibody-arrays-adapted-for-use-with-the-LI-COR-system/biotechniques-305712.html http://www.biotechniques.com/protocols/Multicolor_Tissue_Imaging/New-double-positivity-tool-for-multi-color-IHC-and-IF/biotechniques-305705.html <div style=" background-color: #f6f6f6; padding: 3px; margin: 10px; border: 1px solid #ccc; background-color:#f6f6f6; color: #000; float: right; text-align: left; width:320px; font-size:90%; font:Arial, Helvetica, sans-serif;... Tue, 09 Nov 2010 18:22:53 +0000 http://www.biotechniques.com/protocols/Multicolor_Tissue_Imaging/New-double-positivity-tool-for-multi-color-IHC-and-IF/biotechniques-305705.html http://www.biotechniques.com/protocols/Cell_Culture/An-Analysis-of-Desiccation-in-CO2-Incubators-Using-an-Active-umidification---System-vs.-Water-Pan-Humidity-Generation/biotechniques-305701.html Tue, 09 Nov 2010 18:22:48 +0000 http://www.biotechniques.com/protocols/Cell_Culture/An-Analysis-of-Desiccation-in-CO2-Incubators-Using-an-Active-umidification---System-vs.-Water-Pan-Humidity-Generation/biotechniques-305701.html http://www.biotechniques.com/protocols/High-Throughput_Screening/Lumigen-HyPerBlu-provides-sensitive-chemiluminescent-detection-of-hydrogen---peroxide/biotechniques-305700.html <div style=" background-color: #f6f6f6; padding: 3px; margin: 10px; border: 1px solid #ccc; background-color:#f6f6f6; color: #000; float: right; text-align: left; width:320px; font-size:90%; font:Arial, Helvetica, sans-serif;... Tue, 09 Nov 2010 16:40:45 +0000 http://www.biotechniques.com/protocols/High-Throughput_Screening/Lumigen-HyPerBlu-provides-sensitive-chemiluminescent-detection-of-hydrogen---peroxide/biotechniques-305700.html http://www.biotechniques.com/protocols/RNAi_Protocols/siRNA/Stealth-RNAi-siRNA-brain-infusion/biotechniques-186211.html Materials Mouse (six months old) Reagents: BLOCK-iT™ Alexa Fluor® 555 Red Fluorescent Control. In vitro-validated Stealth RNAi™ siRNA against gene of interest and Stealth RNAi™ siRNA Negative Control. Osmotic ... Mon, 19 Jul 2010 21:15:18 +0100 http://www.biotechniques.com/protocols/RNAi_Protocols/siRNA/Stealth-RNAi-siRNA-brain-infusion/biotechniques-186211.html http://www.biotechniques.com/protocols/RNAi_Protocols/Small_RNA/Transfecting-Silencer-Select-siRNA-into-rat-primary-astrocytes/biotechniques-186210.html Materials Cell culture and transfection 1. Rat primary cortical astrocytes (Invitrogen, Carlsbad, CA, USA) 2. Dulbecco's Modified Eagle Medium (DMEM) high glucose (Invitrogen) supplemented with 15% fetal bovine serum (FBS) (Invitrogen)... Thu, 25 Mar 2010 18:51:40 +0000 http://www.biotechniques.com/protocols/RNAi_Protocols/Small_RNA/Transfecting-Silencer-Select-siRNA-into-rat-primary-astrocytes/biotechniques-186210.html http://www.biotechniques.com/protocols/RNAi_Protocols/microRNA_2/MicroRNA-Profiling-Using-miRCURY-LNA-Array/biotechniques-185911.html This protocol is for a microRNA profile using the miRCURY™ LNA Arrays with the Tecan HS4800™ Pro Hybridization Station. Protocols for manual hybridization are available at Web site (external). Before starting the experiment: ... Mon, 04 Jan 2010 20:33:20 +0000 http://www.biotechniques.com/protocols/RNAi_Protocols/microRNA_2/MicroRNA-Profiling-Using-miRCURY-LNA-Array/biotechniques-185911.html http://www.biotechniques.com/protocols/RNAi_Protocols/siRNA/Producing-siRNA-for-in-vivo-applications/biotechniques-185826.html Background SiRNA is widely used for analyzing gene function and target validation in vitro and in recent years has been successfully extended to in vivo research. Integrated DNA Technologies (IDT) synthesizes siRNAs for both in vitro and in... Mon, 04 Jan 2010 21:06:43 +0000 http://www.biotechniques.com/protocols/RNAi_Protocols/siRNA/Producing-siRNA-for-in-vivo-applications/biotechniques-185826.html http://www.biotechniques.com/protocols/RNAi_Protocols/microRNA_2/MicroRNA-detection-in-FPPE-Sections-by-in-situ-hybridization/biotechniques-185825.html Acknowledgement for the development of these protocols: Dr. Erno Wienholds and Dr. Wigard Kloosterman of the Hubrecht Laboratory, University of Utrecht. Note: the following protocols have been developed using digoxygenin (DIG)-labeled... Thu, 11 Mar 2010 17:16:43 +0000 http://www.biotechniques.com/protocols/RNAi_Protocols/microRNA_2/MicroRNA-detection-in-FPPE-Sections-by-in-situ-hybridization/biotechniques-185825.html http://www.biotechniques.com/protocols/RNAi_Protocols/siRNA/Transfection-of-Caco-2-Cells-with-Using-the-siLentFect-Lipid-Reagent/biotechniques-185812.html The full length version of this protocol, authored by Daniel R. Clayburgh and Jerrold R. Turner, Department of Pathology, The University of Chicago, Chicago, IL 60637, USA, is available from Bio-Rad (bulletin 5370).... Mon, 04 Jan 2010 20:42:10 +0000 http://www.biotechniques.com/protocols/RNAi_Protocols/siRNA/Transfection-of-Caco-2-Cells-with-Using-the-siLentFect-Lipid-Reagent/biotechniques-185812.html http://www.biotechniques.com/protocols/Cell_Culture/Advantages-of-Oxygen-Control-in-CO2-Incubation-for-Research-and----Clinical-Applications/biotechniques-185782.html Physiological in vivo oxygen concentrations can range from 1% to 13%, yet most research and culture is done at ambient 21% O2. Studies have shown dramatic differences in gene expression profiles and phenotypic changes in... Mon, 22 Mar 2010 14:35:32 +0000 http://www.biotechniques.com/protocols/Cell_Culture/Advantages-of-Oxygen-Control-in-CO2-Incubation-for-Research-and----Clinical-Applications/biotechniques-185782.html http://www.biotechniques.com/protocols/Cell_Culture/Inoculation-Growth-and-Harvesting-in-a-New-Easy-to-Use-Disposable-Cell----Culture-System/biotechniques-185491.html Introduction New Brunswick Scientific now offers a new labor-saving device for producing mg quantities of proteins, virus or cell mass from anchorage-dependent or suspension cultures.... Mon, 04 Jan 2010 16:07:37 +0000 http://www.biotechniques.com/protocols/Cell_Culture/Inoculation-Growth-and-Harvesting-in-a-New-Easy-to-Use-Disposable-Cell----Culture-System/biotechniques-185491.html http://www.biotechniques.com/protocols/Cell_Culture/Growing-CHO-cells-in-a-benchtop-stirred-tank-bioreactor-using-single-use----vessels/biotechniques-185490.html Materials Bioreactor (CelliGen BLU; New Brunswick Scientific). Pre-assembled, pre-sterilized vessels include pitched-blade impeller, microsparger, tubing, and more. All components in product contact are made of materials that meet USP... Mon, 19 Jul 2010 21:14:46 +0100 http://www.biotechniques.com/protocols/Cell_Culture/Growing-CHO-cells-in-a-benchtop-stirred-tank-bioreactor-using-single-use----vessels/biotechniques-185490.html http://www.biotechniques.com/protocols/Immunohistochemistry_Paraffin/Whole-Mount-Immunohistochemistry/biotechniques-184555.html Whole mount staining is the staining of small pieces of tissue, usually embryos, without sectioning onto slides first. This is often used on embryos by stem cell and embryonic development researchers and also neuroscientists who are able to stain the... Mon, 04 Jan 2010 15:40:14 +0000 http://www.biotechniques.com/protocols/Immunohistochemistry_Paraffin/Whole-Mount-Immunohistochemistry/biotechniques-184555.html