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BioTechniques' Protocol Guide 2010
is a collection of detailed, optimized procedures/protocols for use in the lab. With BioTechniques' Protocol Guide, you have access to detailed product information in a downloadable PDF format-which can be easily added to a notebook for ready access. Each protocol is accompanied by a link to the company web site and an e-mail link to contact the company directly.
Antibody-based Technologies
Featured Protocols
Antibody-based Technologies
. Vendor-submitted protocol . Published in November 2009
Immunofluorescence
. Vendor-submitted protocol . Sponsored by R&D Systems, Inc. . Published in BioTechniques Protocol Guide 2008
Latest Protocols
Immunofluorescence
(Registration Required)
A key to successful identification of biomolecules by immunofluorescence (IF) staining involves careful selection of specific antibodies. Many of R&D Systems antibodies have been extensively tested for IHC/IF applications and are selected based on a high signal-to-noise ratio.
Multi-analyte Profiling
Greta Wegner, Ph.D.; Benjamin Cook; Christopher Buehl; and Kathy Brumbaugh, Ph.D. Vendor-submitted protocol. Sponsored by R&D Systems, Inc. Published in November 2010
29 - pp
Proteome Profiler Antibody Arrays are rapid, sensitive, and economical assays for the simul­taneous detection of multiple secreted and intracellular proteins. Originally developed for chemiluminescence detection, select arrays can now be adapted for the LI-COR Odyssey® Infrared Imaging System.
Immunostaining
Ricardo B. deMedeiros, Ph.D. and Joy Aho, Ph.D. Vendor-submitted protocol. Sponsored by R&D Systems, Inc. Published in November 2009
Flow cytometry is a powerful technique for assessing the expression of multiple proteins simultaneously in individual cells within a heterogeneous population. This technique is most commonly used with non-adherent cells, but it can also be an effective tool for analyzing protein expression in adherent cells.
Immunofluorescence
Vendor-submitted protocol. Sponsored by Abcam plc Published in BioTechniques Protocol Guide 2010
The ELISPOT assay is a highly sensitive technique to study cell responses to various drugs, stimuli, and inhibitors, and allows the detection of individual cells secreting a particular protein. ELISPOT is often used to investigate the Th1/Th2 response, cancer, vaccine development, viral infection monitoring, infectious diseases, autoimmune diseases, and transplantation, among others.
Vendor-submitted protocol. Published in November 2009
Modern clinical drug development relies on both in vivo and ex vivo imaging to assist in lead qualification, efficacy assessments, toxicity determinations, and biomarker discovery and validation. CRi's Maestro™ and Nuance™ utilize the same multispectral imaging technology to view and quantitate signals from multiple fluorophores bound to targets in small animals or in tissue sections viewed on the microscope.
Immunofluorescence
Vendor-submitted protocol. Sponsored by R&D Systems Published in November 2009
Chromatin proteins play crucial roles in DNA repair, replication, and transcription. Chromatin immunoprecipitation (ChIP) has become a powerful technique in gene expression studies, used to assess whether a certain protein-DNA interaction is present at a given location, condition, and time point.
Antibody Production
Vendor-submitted protocol. Sponsored by STEMCELL Technologies, Inc. Published in BioTechniques Protocol Guide 2009
STEMCELL Technologies’ ClonaCell®-HY media are optimized to support hybridoma selection and growth. Simultaneous hybridoma selection and cloning can be performed in 96-well plates.
Epigenetic Modification Analysis
Vendor-submitted protocol. Sponsored by Assay Designs, Inc. Published in BioTechniques Protocol Guide 2009
Replacement of traditional ELISA with multiplex immunoassay arrays makes the simultaneous measurement of multiple analytes possible while preserving precious sample, resulting in the more cost-effective assessment of biomarkers. The Inflammation (human) 8-Plex MultiBead™ Panel from Assay Designs and corresponding singleplex kits enable the measurement of eicosanoids (PGE2 and TXB2) and inflammatory cytokines (IL-4, IL-6, IL-8, IL-1β, IFN-γ, and TNF-α) in a variety of matrices including tissue culture media, serum, plasma, and urine.
Immunofluorescence
Vendor-submitted protocol. Sponsored by R&D Systems, Inc. Published in BioTechniques Protocol Guide 2008
A key to successful identification of biomolecules by immunofluorescence (IF) staining involves careful selection of specific antibodies. Many of R&D Systems antibodies have been extensively tested for IHC/IF applications and are selected based on a high signal-to-noise ratio.
ELISA
Vendor-submitted protocol. Published in December 2006 2007
The initial cloning and characterization of a Toll-like receptor (TLR) in 1997 has sparked research interests resulting in over 5000 publications to date. Subsequent studies have revealed at least 10 unique TLR receptors exist that respond to unique pathogen-associated molecular patterns.
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