Immunofluorescence
Featured Protocols
Immunofluorescence
. Vendor-submitted protocol . Sponsored by R&D Systems . Published in November 2009
Chromatin proteins play crucial roles in DNA repair, replication, and transcription...
Immunofluorescence
. Vendor-submitted protocol . Sponsored by R&D Systems, Inc. . Published in BioTechniques Protocol Guide 2008
Latest Protocols
Staining of Rat Cortical Stem Cells
(Registration Required)
A key to successful identification of biomolecules by immunofluorescence (IF) staining involves careful selection of specific antibodies. Many of R&D Systems antibodies have been extensively tested for IHC/IF applications and are selected based on a high signal-to-noise ratio.
ELISPOT and Dual ELISPOT: a cell-based assay to assess immune response
(Registration Required)
Vendor-submitted protocol. Sponsored by Abcam plc Published in BioTechniques Protocol Guide 2010
The ELISPOT assay is a highly sensitive technique to study cell responses to various drugs, stimuli, and inhibitors, and allows the detection of individual cells secreting a particular protein. ELISPOT is often used to investigate the Th1/Th2 response, cancer, vaccine development, viral infection monitoring, infectious diseases, autoimmune diseases, and transplantation, among others.
Chromatin Immunoprecipitation to Measure Transcription Factor–DNA Interactions
Vendor-submitted protocol. Sponsored by R&D Systems Published in November 2009
Chromatin proteins play crucial roles in DNA repair, replication, and transcription. Chromatin immunoprecipitation (ChIP) has become a powerful technique in gene expression studies, used to assess whether a certain protein-DNA interaction is present at a given location, condition, and time point.
Staining of Rat Cortical Stem Cells
Vendor-submitted protocol. Sponsored by R&D Systems, Inc. Published in BioTechniques Protocol Guide 2008
A key to successful identification of biomolecules by immunofluorescence (IF) staining involves careful selection of specific antibodies. Many of R&D Systems antibodies have been extensively tested for IHC/IF applications and are selected based on a high signal-to-noise ratio.


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