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BioTechniques' Protocol Guide 2010
is a collection of detailed, optimized procedures/protocols for use in the lab. With BioTechniques' Protocol Guide, you have access to detailed product information in a downloadable PDF format-which can be easily added to a notebook for ready access. Each protocol is accompanied by a link to the company web site and an e-mail link to contact the company directly.
protocol
Mice to Microscopy: Fluorescence In Vivo and Ex Vivo Imaging of the Same Animal
Sponsored,vendor-submitted protocol   Published in November 2009 (p.35) DOI: 10.2144/000113029

Modern clinical drug development relies on both in vivo and ex vivo imaging to assist in lead qualification, efficacy assessments, toxicity determinations, and biomarker discovery and validation. CRi's Maestro™ and Nuance™ utilize the same multispectral imaging technology to view and quantitate signals from multiple fluorophores bound to targets in small animals or in tissue sections viewed on the microscope. This can greatly assist long-term tracking of disease progression in individual animals and can result in increased throughput and productivity.

Synopsis

Fluorescence imaging permits the use of a wide range of probes, labeling methods, and targets, and can even be used with labels that emit in the near-infrared, the spectral “sweet spot” for deep tissue in vivo imaging. The Maestro in vivo imaging system pioneered multispectral approaches to small animal imaging. The system uses solid-state liquid crystal tunable filters (LCTFs), a technology that has been described elsewhere and compared in some detail to other spectral imaging techniques. The Nuance imaging system uses the same technology as the Maestro system, except that it mounts onto any microscope equipped with a standard C-mount adapter.

Figure 1 shows striking results when different lymphatic drainage patterns are individually highlighted by appropriately located injections with different quantum dot species for each region. Figure 2 illustrates the use of presumptively active and inactive molecular reagents in in vivo imaging.





Correspondence

Address correspondence to CRi, 35-B Cabot Road, Woburn, MA 01801 USA, Tel.: 1-800-383-7924, Fax: +1-781-935-3388; [email protected], www.cri-inc.com/bt2008.

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