to BioTechniques free email alert service to receive content updates.

TissueScan qPCR Array: Biomarker Validation via Gene Expression Profiling in a Large Number of Tumors
Sponsored,vendor-submitted protocol   Sponsored by Origene Technologies    Published in November 2007 (p.11)


TissueScan is a unique qPCR array of first-strand cDNA from hundreds of clinical tumor samples. It provides a fast and accurate gene expression profiling across a large number of cancer patient samples. High-quality cDNAs were prepared from highly-documented cancer biopsy samples, normalized and assembled into ready-to-use gene expression panels. This alleviates for researchers the tedious work of large sample collection and the meticulous work of RNA/cDNA preparation, while facilitating quick and reliable profiling of gene expression levels across cancer progression stages. TissueScan combines the high sensitivity and specificity of the RT-PCR methodology with a well-designed multisample format. It is an excellent tool for validation of potential cancer markers such as those obtained by microarray or differential display. Application Example:

To illustrate the validity of TissueScan, we used Lung Cancer Panel (HLRT-101) to evaluate the expression level of Topoisomerase ll alpha, which has been known to be upregulated in a large percentage of lung cancers. A pair of primers was designed to detect the transcript of this gene. SYBR Green I based real-time PCR was performed. The raw data (Figure A) and the expression level plot calculated by the Ct method (Figure B) are shown at right. A second panel in the kit was used with beta-action control primers provided in the kit (Figure C).

(Click to enlarge)

(Click to enlarge)

(Click to enlarge)

The results showed that TOPOII expression levels are markedly higher than in the normal tissues, supporting the notion that topoisomerase can serve as a valid biomarker for lung cancer.

Product Offering:

TissueScan kit includes two identical 48-well panels, beta-actin control primers and a copy of the Application Guide. The normalized cDNAs are lyophilized in 48-well PCR plates.

For more information, contact us at 1-888-267-4436, [email protected], or visit Web site (external).

More Information
Do you have question about this protocol? Would you like more information about this protocol?
Submit your questions here and Biotechniques will forward your question and contact information from our database to an expert at Origene Technologies who will contact you.
Yes, please forward my question, along with my name, title, company, and e-mail address to Origene Technologies
BioTechniques will forward your question to the company, who will contact you directly by email.
Request more information
Name Question
Job Title
Contact Information
rightcontent.jsp called otherwise condition rightcontent.jsp called 222222222222222222222
Search Protocols
Search our protocols for your specific technique.
Submit Your Protocols
BioTechniques is always looking for author-submitted protocols related to peer-reviewed papers, so submit your protocols today to our editorial team. Suppliers or advertisers interested in publishing their protocols should also submit.
Click here for more information