TissueScan is a unique qPCR array of first-strand cDNA from hundreds of clinical tumor samples. It provides a fast and accurate gene expression profiling across a large number of cancer patient samples. High-quality cDNAs were prepared from highly-documented cancer biopsy samples, normalized and assembled into ready-to-use gene expression panels. This alleviates for researchers the tedious work of large sample collection and the meticulous work of RNA/cDNA preparation, while facilitating quick and reliable profiling of gene expression levels across cancer progression stages. TissueScan combines the high sensitivity and specificity of the RT-PCR methodology with a well-designed multisample format. It is an excellent tool for validation of potential cancer markers such as those obtained by microarray or differential display. Application Example:
To illustrate the validity of TissueScan, we used Lung Cancer Panel (HLRT-101) to evaluate the expression level of Topoisomerase ll alpha, which has been known to be upregulated in a large percentage of lung cancers. A pair of primers was designed to detect the transcript of this gene. SYBR Green I based real-time PCR was performed. The raw data (Figure A) and the expression level plot calculated by the Ct method (Figure B) are shown at right. A second panel in the kit was used with beta-action control primers provided in the kit (Figure C).
The results showed that TOPOII expression levels are markedly higher than in the normal tissues, supporting the notion that topoisomerase can serve as a valid biomarker for lung cancer.
TissueScan kit includes two identical 48-well panels, beta-actin control primers and a copy of the Application Guide. The normalized cDNAs are lyophilized in 48-well PCR plates.
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