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TissueScan qPCR Array: Biomarker Validation via Gene Expression Profiling in a Large Number of Tumors
Sponsored,vendor-submitted protocol   Sponsored by Origene Technologies    Published in November 2007 (p.11)

Introduction

TissueScan is a unique qPCR array of first-strand cDNA from hundreds of clinical tumor samples. It provides a fast and accurate gene expression profiling across a large number of cancer patient samples. High-quality cDNAs were prepared from highly-documented cancer biopsy samples, normalized and assembled into ready-to-use gene expression panels. This alleviates for researchers the tedious work of large sample collection and the meticulous work of RNA/cDNA preparation, while facilitating quick and reliable profiling of gene expression levels across cancer progression stages. TissueScan combines the high sensitivity and specificity of the RT-PCR methodology with a well-designed multisample format. It is an excellent tool for validation of potential cancer markers such as those obtained by microarray or differential display. Application Example:

To illustrate the validity of TissueScan, we used Lung Cancer Panel (HLRT-101) to evaluate the expression level of Topoisomerase ll alpha, which has been known to be upregulated in a large percentage of lung cancers. A pair of primers was designed to detect the transcript of this gene. SYBR Green I based real-time PCR was performed. The raw data (Figure A) and the expression level plot calculated by the Ct method (Figure B) are shown at right. A second panel in the kit was used with beta-action control primers provided in the kit (Figure C).


(Click to enlarge)



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The results showed that TOPOII expression levels are markedly higher than in the normal tissues, supporting the notion that topoisomerase can serve as a valid biomarker for lung cancer.

Product Offering:

TissueScan kit includes two identical 48-well panels, beta-actin control primers and a copy of the Application Guide. The normalized cDNAs are lyophilized in 48-well PCR plates.

For more information, contact us at 1-888-267-4436, [email protected], or visit Web site (external).





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