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Cell Culture
Latest Protocols
Michele Bruder, Jeff Krol, Linda Zhang, and George Wang Vendor-submitted protocol. Sponsored by New Brunswick Scientific Published in November 2010
Humidity, temperature, and gas uniformity within a CO2 incubator are the three most important factors to ensuring high yields in cell culture. When humidity is not properly circulated or generated evenly throughout the chamber, the effects can include decreases in cell weight, cell morphology variability, nutrient over-digestion, and decreased protein production.
Vendor-submitted protocol. Sponsored by New Brunswick Scientific Published in BioTechniques 2010 Protocol Guide
This protocol describes setup and operation of New Brunswick Scientific’s new CelliGen™ BLU stirred-tank bioreactor, which combines the accurate process control and trusted performance of traditional stirred-tank technology with the convenience of single-use, pre-sterilized vessels to eliminate autoclaving and cleaning. A procedure is described for culturing Chinese hamster ovarian (CHO) cells in a 5.0-L vessel, using CD CHO serum-free medium in a batch culture.
Vendor-submitted protocol. Sponsored by New Brunswick Scientific Published in BioTechniques Protocol Guide 2009
Physiological in vivo oxygen concentrations can range from 1% to 13%, yet most research and culture is done at ambient 21% O2. Studies have shown dramatic differences in gene expression profiles and phenotypic changes in the cells between these conditions.
Vendor-submitted protocol. Sponsored by Affymetrix, Inc Published in November 2007
The Affymetrix Whole-transcript Sense Target Labeling Assay is designed to generate unbiased, amplified and biotinylated sense-strand DNA targets from the entire expressed genome. Using the Whole-transcript Assay in combination with GeneChip® Exon 1.0 ST and Gene 1.0 ST Arrays allows you to measure expression across the entire length of a gene, thus offering a more complete and accurate picture of overall gene expression than 3'-based assays.
Vendor-submitted protocol. Published in November 2006
New Brunswick Scientific now offers a new labor-saving device for producing mg quantities of proteins, virus or cell mass from anchorage-dependent or suspension cultures. More productive than traditional roller bottles, spinners or T-flasks, the new FibraStage system is inexpensive, simple to operate, and disposable.
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