Michele Bruder, Jeff Krol, Linda Zhang, and George Wang
New Brunswick Scientific
Humidity, temperature, and gas uniformity within a CO2 incubator are the three
most important factors to ensuring high yields in cell culture. When
humidity is not properly circulated or generated evenly throughout the
chamber, the effects can include decreases in cell weight, cell morphology
variability, nutrient over-digestion, and decreased protein production.
This protocol describes setup and operation of New Brunswick Scientific’s new CelliGen™ BLU stirred-tank bioreactor, which combines the accurate process control and trusted performance of traditional stirred-tank technology with the convenience of single-use, pre-sterilized vessels to eliminate autoclaving and cleaning. A procedure is described for culturing Chinese hamster ovarian (CHO) cells in a 5.0-L vessel, using CD CHO serum-free medium in a batch culture.
Physiological in vivo oxygen concentrations can range from 1% to 13%, yet most research and culture is done at ambient 21% O2. Studies have shown dramatic differences in gene expression profiles and phenotypic changes in the cells between these conditions.
The Affymetrix Whole-transcript Sense Target Labeling Assay is designed to generate unbiased, amplified and biotinylated sense-strand DNA targets from the entire expressed genome. Using the Whole-transcript Assay in combination with GeneChip® Exon 1.0 ST and Gene 1.0 ST Arrays allows you to measure expression across the entire length of a gene, thus offering a more complete and accurate picture of overall gene expression than 3'-based assays.
New Brunswick Scientific now offers a new labor-saving device for producing mg quantities of proteins, virus or cell mass from anchorage-dependent or suspension cultures. More productive than traditional roller bottles, spinners or T-flasks, the new FibraStage system is inexpensive, simple to operate, and disposable.
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