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An Analysis of Desiccation in CO2 Incubators Using an Active umidification System vs. Water-Pan Humidity Generation
Michele Bruder, Jeff Krol, Linda Zhang, and George Wang
Sponsored,vendor-submitted protocol    Sponsored by New Brunswick Scientific    Published in November 2010 DOI: 10.2144/000113571


Humidity, temperature, and gas uniformity within a CO2 incubator are the three most important factors to ensuring high yields in cell culture. When humidity is not properly circulated or generated evenly throughout the chamber, the effects can include decreases in cell weight, cell morphology variability, nutrient over-digestion, and decreased protein production. 1, 2, 3, 4

This study evaluated growth of Chinese Hamster Ovary (CHO) cells in a New Brunswick Scientific Galaxy┬« CO2 Incubator equipped with an Active Humidification System (AHS) to study this system’s efficacy against a competitive unit with traditional water-pan humidity. The incubator doors were repeatedly opened for two-minute periods throughout the study to mimick conditions encountered in a typical cell culture lab. Desiccation was measured by comparing individual flask weights prior to incubation and at the conclusion of each study. The data clearly shows that the Galaxy incubator’s Active Humidification System provided rapid and uniform humidity recovery, minimizing the potentially deliterious desiccation effect.

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