2012 Protocol Guide
Traditional cloning procedures with restriction enzymes have limitations and lack efficiency when constructing recombinant molecules using multiple fragments. SA-cloning can construct an expression plasmid over 10kb with multiple fragments at one time by the self-assembly of complementary staggered overhangs on PCR-amplified fragments.
2012 Protocol Guide
Differential amplification of variant and wild-type alleles by PCR is often used for rare allele enrichment. We have combined allele-specific PCR, competitive probe blocking, asymmetric PCR, and melting analysis to enhance rare allele detection in a homogeneous system.
2012 Protocol Guide
⇒ATTENTION *HINT ✋REST Procedure Cleavage of surface proteins of cultured cells 1. Wash the cells expressing your protein of interest with phosphate buffered saline (PBS) on ice.
2012 Protocol Guide
We have developed a quick and low-cost genomic DNA extraction protocol from yeast cells for PCR-based applications. This method does not require any enzymes, hazardous chemicals, or extreme temperatures, and is especially powerful for simultaneous analysis of a large number of samples.
2012 Protocol Guide
The development of automated microscopy platforms has enabled large-scale observation of biological processes, thereby complementing genome scale biochemical techniques. However, commercially available systems are restricted either by fixed field-of-views, leading to potential omission of features of interest, or by low-resolution data of whole objects lacking cellular detail.
2012 Protocol Guide
R&D Systems Cell-Based Infrared ELISAs simultaneously measure the levels of two proteins in the same microplate well in whole cells, eliminating the need for lysate preparation. Kits utilize LI-COR® IRDye® 800CW- and IRDye® 680LT-conjugated secondary antibodiesaaThis product is covered by US 6,995,274; US 7,504,089; foreign equivalents and patents pendingto detect the protein of interest and a second housekeeping protein in adherent or non-adherent cells, allowing for normalization of the target protein in each well.
Protocols
Vendor-submitted protocol.
Sponsored by
Sequenom
Tumor genotyping can provide a useful guide to drive clinical trials, inform
treatment options, and predict patient outcomes1. This is due, in large
part, to our understanding of major cancer pathways and their use in
therapeutic strategies.
Small RNA
Vendor-submitted protocol.
Sponsored by
Applied Biosystems, part of Life Technologies
Published in
BioTechniques 2010 Protocol Guide
Lipofectamine® RNAiMAX Transfection Reagent is a proprietary, animal
origin–free formulation for the transfection of siRNA into eukaryotic cells
with low cytotoxicity. Silencer® Select siRNAs are high-performing
siRNA molecules that incorporate the latest innovations in siRNA design,
chemical modifications, and off-target effect prediction algorithms.
Peer-Reviewed Protocols
Wai Hung Tsang and King L. Chow
Peer-reviewed, author-submitted protocol.
Published in
November
2009
pp55-
56
Procedure 1. A pair of fine forceps with a region of ~1 mm in width is used to hold the thin end of an autoclaved gel-loading tip in a perpendicular position (Figure 1).
Peer-Reviewed Protocols
Kim M. Linton1,5*, Yvonne Hey2*, Sian Dibben2, Crispin J. Miller3, Anthony J. Freemont4, John A. Radford1,5, and Stuart D. Pepper2
Peer-reviewed, author-submitted protocol.
Published in
November
2009
Protocol overview
• This protocol covers tissue sectioning and macrodissection steps in preparation for RNA extraction from FFPE tissues. Although originally developed for RNA extraction using the Optimum FFPE RNA Isolation Kit (Ambion, UK), the protocol is relevant for FFPE tissue preparation for RNA extraction using any extraction method
• Macrodissection is carried out at 2× magnification using a dissecting microscope with external fiber-optic illumination
• Adopt strict RNase-free handling conditions throughout, including the use and frequent changing of powder-free latex gloves.