Small RNA
Vendor-submitted protocol.
Sponsored by
Applied Biosystems, part of Life Technologies
Published in
BioTechniques 2010 Protocol Guide
45
-
pp
Lipofectamine® RNAiMAX Transfection Reagent is a proprietary, animal
origin–free formulation for the transfection of siRNA into eukaryotic cells
with low cytotoxicity
Peer-Reviewed Protocols
Wai Hung Tsang and King L. Chow
Peer-reviewed, author-submitted protocol.
Published in
November
2009
pp55-
56
Peer-Reviewed Protocols
Kim M. Linton1,5*, Yvonne Hey2*, Sian Dibben2, Crispin J. Miller3, Anthony J. Freemont4, John A. Radford1,5, and Stuart D. Pepper2
Peer-reviewed, author-submitted protocol.
Published in
November
2009
Peer-Reviewed Protocols
Kim M. Linton1,5*, Yvonne Hey2*, Sian Dibben2, Crispin J. Miller3, Anthony J. Freemont4, John A. Radford1,5, and Stuart D. Pepper2
Peer-reviewed, author-submitted protocol.
Published in
November
2009
Peer-Reviewed Protocols
Michael A. Green1, Shannon Bass2, and Brett T. Spear1,3
Peer-reviewed, author-submitted protocol.
Published in
November
2009
Peer-Reviewed Protocols
Yong-Chul Jung1, Jia Xu2, Jun Chen1, Yeong C. Kim1, David J. Winchester3, and San Ming Wang1
Peer-reviewed, author-submitted protocol.
Published in
November
2009
pp46-
47
Depending on the desired resolution and sequencing scale, different restriction enzymes can be used for genomic DNA digestion. PstI is used here as an example
From Soil/Environmental Samples
Vendor-submitted protocol.
Sponsored by
Zymo Research Corporation
Published in
November
2009
Metagenomics (otherwise known as environmental genomics, ecogenomics, or community genomics) is the study of genetic material recovered directly from environmental samples (e.g., soil)
Methylation Detection
Vendor-submitted protocol.
Sponsored by
Zymo Research Corporation
Published in
November
2009
High Resolution Melt (HRM) analysis can be used to differentiate DNAs based on variations in primary sequence for applications such as genotyping, SNP analysis, and DNA methylation analysis
Immunostaining
Ricardo B. deMedeiros, Ph.D. and Joy Aho, Ph.D.
Vendor-submitted protocol.
Sponsored by
R&D Systems, Inc.
Published in
November
2009
Flow cytometry is a powerful technique for assessing the expression of multiple proteins simultaneously in individual cells within a heterogeneous population
Protein Extraction Isolation and Purification
Vendor-submitted protocol.
Sponsored by
Promega Corporation
The HaloTag® Protein Purification System is based on a unique protein tag, HaloTag®, which allows covalent, efficient, and specific capture of proteins expressed in Escherichia coli
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