Confirmation of pluripotency is a critical experimental step when studying human embryonic and/or induced pluripotent stem cells. To ensure consistency between studies, it is important to verify the maintenance of undifferentiated cells in culture.
BioTechniques Protocol Guide 2007
Laser Capture Microdissection (LCM) is a proven technique for isolating pure cell populations for downstream molecular analysis. Combined UV laser cutting and LCM using the IR laser, found only with the Veritas™ Microdissection System, allows for rapid and precise isolation of larger numbers of cells, while maintaining cellular and nucleic acid integrity necessary for downstream analysis.
Somatic Cell Nuclear Transfer (SCNT) is the process of removing from an unfertilized oocyte its own chromosomes (enucleation) and replacing them with chromosomes taken from a somatic cell. Parthenogenesis may then be induced in order to create an embryonic clone of the donor of the somatic DNA, with either the intent of producing a live-birth clone, or of producing embryonic stem cells compatible with the somatic cell donor.
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