Flow cytometry is the technique of choice for analysis of mitochondrial transmembrane potential (ΔΨm) in whole cells. JC-1, a cytofluorimetric, lipophilic cationic dye, selectively enters into mitochondria and reversibly changes color from red to green as the membrane potential decreases.
The Accuri C6 Flow Cytometer System is an ideal platform on which to perform no-wash, differential analysis of human peripheral blood samples. The fixed-voltage PMTs simplify data collection and reduce the potential for data loss due to improper signal amplification.
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